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crystal structure of the bifunctional leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase, the binary complex structure with NADP+, and the ternary complex structure with NADP+ and 15-oxo-prostaglandin E2, batch method using the automated crystallization system TERA by mixing equal volumes of protein solution (20 mM Tris-HCL, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol) and precipitating solution (100 mM 4-morpholineethanesulfonic acid, pH 5.5-6.8), 17.5-27.5% polyethylene glycol 4000, 50 mM MgCl2 at 18°C
leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase complex in ternary complex with indomethacin and NADP+. Indomethacin binds in anti-configuration at one of the active side clefts of the homodimer interface of the enzyme. The chlorobenzene is buried in the hydrophobic pore and the carboxyl group interacts with the guanidino group of R56 and the phenolic hydroxyl group of Y262