1.3.1.34: 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

This is an abbreviated version!
For detailed information about 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing], go to the full flat file.

Word Map on EC 1.3.1.34

Reaction

a (2E)-2-enoyl-CoA
+
NADP+
=
a (2E,4E)-2,4-dienoyl-CoA
+
NADPH
+
H+

Synonyms

2,4-dienoyl coenzyme A reductase, 2,4-dienoyl-CoA reductase, 2,4-dienoyl-CoA reductase (NADPH), 2,4-dienoyl-CoA reductase [NADPH], 4-enoyl-CoA reductase (NADPH), 4-enoyl-CoA reductase [NADPH], DCR, DECR, pDCR, peroxisomal 2,4-dienoyl CoA reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.34 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

Purification

Purification on EC 1.3.1.34 - 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
dye-ligand chromatography, general ligand affinity-chromatography
-
gel filtration
-
ion-exchange chromatography
-
ion-exchange chromatography or dye-ligand chromatography, affinity chromatography
-
Ni-NTA chromatography
NTA-Ni2+ agarose column chromatography
partial, solubilization of enzyme using Triton X-100 from heart mitochondria
-
recombinant enzyme from strain BL21(DE3) via DEAE ion exchange and affinity chromatography
-
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity
-