1.3.1.34: 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

This is an abbreviated version!
For detailed information about 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing], go to the full flat file.

Word Map on EC 1.3.1.34

Reaction

a (2E)-2-enoyl-CoA
+
NADP+
=
a (2E,4E)-2,4-dienoyl-CoA
+
NADPH
+
H+

Synonyms

2,4-dienoyl coenzyme A reductase, 2,4-dienoyl-CoA reductase, 2,4-dienoyl-CoA reductase (NADPH), 2,4-dienoyl-CoA reductase [NADPH], 4-enoyl-CoA reductase (NADPH), 4-enoyl-CoA reductase [NADPH], DCR, DECR, pDCR, peroxisomal 2,4-dienoyl CoA reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.34 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

Engineering

Engineering on EC 1.3.1.34 - 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C334A/C337A
the mutation exhibits 0.02% of wild type activity
C337A
the mutation results in the loss of most of the iron and acid-labile sulfur and exhibits 0.09% of wild type activity
E164A
mutant exhibits 1.5% of the wild type activity
H252A
the mutation exhibits 0.09% of wild type activity
H252F
mutant is inactive
Y166F
mutant exhibits 27% of the wild type activity, however, the product of the reduction is 3-enoyl-CoA instead of 2-enoyl-CoA
Y166F/E164Q
double mutant is inactive
Y166F/H252A
the double mutation exhibits 4.4% of the wild type activity
D137A
relative activity: 3.32% (substrate: 2,4-decadienoyl CoA), 1.27% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)
D155A
relative activity: 36.3% (substrate: 2,4-decadienoyl CoA), 59.6% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)
D186A
relative activity: 6.21% (substrate: 2,4-decadienoyl CoA), 1.86% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)
D268A
relative activity: 2.75% (substrate: 2,4-decadienoyl CoA), 1.14% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)
D86A
relative activity: 1.70% (substrate: 2,4-decadienoyl CoA), 1.76% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)
E215A
relative activity: 28.9% (substrate: 2,4-decadienoyl CoA), 7.59% (substrate: 2,4-hexadienoyl CoA) (wild-type: 100%)