1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase

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For detailed information about DELTA4-3-oxosteroid 5beta-reductase, go to the full flat file.

Word Map on EC 1.3.1.3

1.3.1.3

bile

chenodeoxycholic

digitalis

akr1c4

cardenolide

lanata

bile-acids

cyp7b1

delta4-3-ketosteroids

tocolytic

srd5a1

pre-receptor

medicine

Reaction

17,21-dihydroxy-5beta-pregnane-3,11,20-trione

Synonyms

3-oxo-DELTA4-steroid 5beta-reductase, 4,5beta-dihydrocortisone:NADP+ DELTA4-oxidoreductase, 5-beta-reductase, 5beta-POR, 5beta-red, AKR1D1, AKR1D4, aldo-keto reductase, aldo-keto reductase 1D1, aldo�keto reductase 1D1, androstenedione 5beta-reductase, CARUB_v10005016mg, cortisone 5beta-reductase, cortisone beta-reductase, DELTA 4-5beta-reductase, DELTA4-3-ketosteroid 5beta-reductase, DELTA4-3-oxosteroid 5beta-reductase, DELTA4-hydrogenase, EC 1.3.1.23, EC 1.3.1.3, h5beta-red, h5beta-reductase, More, P5betaR, progesterone 5beta-reductase, reductase, cholestenone 5beta.-, reductase, cortisone DELTA4-5beta-, SRD5beta, St5betaR1, steroid 5beta-reductase, steroid 5beta.-reductase, testosterone 5-beta-reductase, testosterone 5beta-reductase

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.1.3 DELTA4-3-oxosteroid 5beta-reductase

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Results	in table
37	AA Sequence
5	Application
2	CAS Registry Number
20	Cloned(Commentary)
27	Cofactor
9	Crystallization (Commentary)
96	Disease/ Diagnostics
6	Expression
11	General Information
4	General Stability
30	Inhibitors
43	kcat/KM [mM/s]
5	Ki Value [mM]
78	KM Value [mM]
9	Localization
7	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
45	Organism
6	Pathway
34	PDB ID
14	pH Optimum
3	pH Range
1	pH Stability
1	pI Value
49	Protein Variants
18	Purification (Commentary)
6	Reaction
6	Reaction Type
43	Reference
29	Source Tissue
10	Specific Activity [micromol/min/mg]
4	Storage Stability
123	Substrates and Products (Substrate)
7	Subunits
67	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Stability [°C]
53	Turnover Number [1/s]

Purification

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Purification on EC 1.3.1.3 - DELTA4-3-oxosteroid 5beta-reductase

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3 entries

by Ni-NTA affinity chromatography

2 entries

cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein

Rattus norvegicus

390590, 390591

glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration

Homo sapiens

P51857

685271

Ni2+-Sepharose column chromatography

Homo sapiens

687723

nickel-Sepharose affinity column chromatography and Superdex 75 gel filtration

partial

recombinant AKR1D1 enzyme mutant P133R from HEK-293 cells, the other expressed mutants cannot be purified

Homo sapiens

P51857

725399

recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity and anion exchange chromatography, and gel filtration

recombinant protein

recombinant wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography

Arabidopsis thaliana

Q9STX2

726120

to apparent homogeneity by affinity chromatography

Digitalis purpurea

671346

to homogeneity by NiNTA affinity chromatography

Digitalis lanata

671101