1.3.1.2: dihydropyrimidine dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about dihydropyrimidine dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.3.1.2

Reaction

5,6-dihydrouracil
+
NADP+
=
Uracil
+
NADPH
+
H+

Synonyms

4,5-dihydrothymine: oxidoreductase, dehydrogenase, dihydrouracil (nicotinamide adenine dinucleotide phosphate), DHPDH, DHPDHase, DHU dehydrogenase, dihydropyrimidine dehydrogenase, dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, dihydrouracil dehydrogenase (NADP), dihydrouracil dehydrogenase (NADP+), DPD, DPYD, hydropyrimidine dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.2 dihydropyrimidine dehydrogenase (NADP+)

Reference

Reference on EC 1.3.1.2 - dihydropyrimidine dehydrogenase (NADP+)

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Traut, T.W.; Loechel, S.
Pyrimidine catabolism: individual characterization of the three sequential enzymes with a new assay
Biochemistry
23
2533-2539
1984
Rattus norvegicus
Manually annotated by BRENDA team
Hallock, R.O.; Yamada, E.W.
Pyrimidine reducing enzymes of rat liver
Can. J. Biochem.
54
178-184
1976
Rattus norvegicus
Manually annotated by BRENDA team
Haaz, M.C.; Fischel, J.L.; Formento, P.; Renee, N.; Etienne, M.C.; Milano, G.
Impact of different fluorouracil biochemical modulators on cellular dihydropyrimidine dehydrogenase
Cancer Chemother. Pharmacol.
38
52-58
1996
Homo sapiens
Manually annotated by BRENDA team
Milano, G.; Fischel, J.L.; Etienne, M.C.; Renee, N.; Formento, P.; Thyss, A.; Gaspard, M.H.; Thill, L.; Cupissol, D.
Inhibition of dihydropyrimidine dehydrogenase by alpha-interferon: Experimental data on human tumor cell lines
Cancer Chemother. Pharmacol.
34
147-152
1994
Homo sapiens
Manually annotated by BRENDA team
Fritzson, P.
Properties and assay of dihydrouracil dehydrogenase of rat liver
J. Biol. Chem.
235
719-725
1960
Rattus norvegicus
Manually annotated by BRENDA team
Grisolia, S.; Cardoso, S.S.
The purification and properties of hydropyrimidine dehydrogenase
Biochim. Biophys. Acta
25
430-431
1957
Bos taurus
Manually annotated by BRENDA team
Shiotani, T.; Weber, G.
Purification and properties of dihydrothymine dehydrogenase from rat liver
J. Biol. Chem.
256
219-224
1981
Rattus norvegicus
Manually annotated by BRENDA team
Tuchman, M.; Roemeling, R.V.; Hrushesky, W.A.M.; Sothern, R.B.; O'Dea, R.F.
Dihydropyrimidine dehydrogenase activity in human blood mononuclear cells
Enzyme
42
15-24
1989
Homo sapiens
Manually annotated by BRENDA team
Klein, C.M.; Haas, H.J.
New high-performance liquid chromatographic method for the determination of low activities of dihydropyrimidine dehydrogenase
J. Chromatogr.
529
431-436
1990
Rattus norvegicus
Manually annotated by BRENDA team
Porter, D.J.T.; Chestnut, W.G.; Merrill, B.M.; Spector, T.
Mechanism-based inactivation of dihydropyrimidine dehydrogenase by 5-ethynyluracil
J. Biol. Chem.
267
5236-5242
1992
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Podschun, B.; Wahler, G.; Schnackerz, K.D.
Purification and characterization of dihydropyrimidine dehydrogenase from pig liver
Eur. J. Biochem.
185
219-224
1989
Sus scrofa
Manually annotated by BRENDA team
Podschun, B.; Cook, P.F.; Schnackerz, K.D.
Kinetic mechanism of dihydropyrimidine dehydrogenase from pig liver
J. Biol. Chem.
265
12966-12972
1990
Sus scrofa
Manually annotated by BRENDA team
Podschun, B.
Stereochemistry of NADPH oxidation by dihydropyrimidine dehydrogenase from pig liver
Biochem. Biophys. Res. Commun.
182
609-616
1992
Sus scrofa
Manually annotated by BRENDA team
Porter, D.J.T.; Chestnut, W.G.; Taylor, L.C.E.; Merrill, B.M.; Spector, T.
Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil
J. Biol. Chem.
266
19988-19994
1991
Bos taurus
Manually annotated by BRENDA team
Balzarini, J.; Sienaert, R.; Liekens, S.; Van Kuilenburg, A.; Carangio, A.; Esnouf, R.; De Clercq, E.; McGuigan, C.
Lack of susceptibility of bicyclic nucleoside analogs, highly potent inhibitors of varicella-zoster virus, to the catabolic action of thymidine phosphorylase and dihydropyrimidine dehydrogenase
Mol. Pharmacol.
61
1140-1145
2002
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Schmitt, U.; Jahnke, K.; Rosenbaum, K.; Cook, P.F.; Schnackerz, K.D.
Purification and characterization of dihydropyrimidine dehydrogenase from Alcaligenes eutrophus
Arch. Biochem. Biophys.
332
175-182
1996
Cupriavidus necator
Manually annotated by BRENDA team
Goudgaon, N.M.; Naguib, F.N.M.; el Kouni, M.H.; Schinazi, R.F.
Phenylselenenyl- and phenylthio-substituted pyrimidines as inhibitors of dihydrouracil dehydrogenase and uridine phosphorylase
J. Med. Chem.
36
4250-4254
1993
Mus musculus
Manually annotated by BRENDA team
Naguib, F.N.M.; El Kouni, M.H.; Cha, S.
Structure-activity relationship of ligands of dihydrouracil dehydrogenase from mouse liver
Biochem. Pharmacol.
38
1471-1480
1989
Mus musculus
Manually annotated by BRENDA team
Rosenbaum, K.; Jahnke, K.; Curti, B.; Hagen, W.R.; Schnackerz, K.D.; Vanoni, M.A.
Porcine Recombinant Dihydropyrimidine Dehydrogenase: Comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes
Biochemistry
37
17598-17609
1998
Sus scrofa
Manually annotated by BRENDA team
Lu, Z.H.; Zhang, R.; Diasio, R.B.
Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties
Biochem. Pharmacol.
46
945-952
1993
Bos taurus, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Rosenbaum, K.; Schaffrath, B.; Hagen, W.R.; Jahnke, K.; Gonzalez, F.J.; Cook, P.F.; Schnackerz, K.D.
Purification, characterization, and kinetics of porcine recombinant dihydropyrimidine dehydrogenase
Protein Expr. Purif.
10
185-191
1997
Sus scrofa
Manually annotated by BRENDA team
Van Kuilenburg, A.B.P.; Van Lenthe, H.; Wanders, R.J.A.; Van Gennip, A.H.
Subcellular localization of dihydropyrimidine dehydrogenase
Biol. Chem.
378
1047-1053
1997
Rattus norvegicus
Manually annotated by BRENDA team
Hagen, W.R.; Vanoni, M.A.; Rosenbaum, K.; Schnackerz, K.D.
On the iron-sulfur clusters in the complex redox enzyme dihydropyrimidine dehydrogenase
Eur. J. Biochem.
267
3640-3646
2000
Sus scrofa
Manually annotated by BRENDA team
Dobritzsch, D.; Ricagno, S.; Schneider, G.; Schnackerz, K.D.; Lindqvist, Y.
Crystal structure of the productive ternary complex of dihydropyrimidine dehydrogenase with NADPH and 5-iodouracil. Implications for mechanism of inhibition and electron transfer
J. Biol. Chem.
277
13155-13166
2002
Sus scrofa
Manually annotated by BRENDA team
Porter, D.J.T.; Spector, T.
Dihydropyrimidine dehydrogenase. Kinetic mechanism for reduction of uracil by NADPH
J. Biol. Chem.
268
19321-19327
1993
Bos taurus
Manually annotated by BRENDA team
Podschun, B.; Jahnke, K.; Schnackerz, K.D.; Cook, P.F.
Acid base catalytic mechanism of the dihydropyrimidine dehydrogenase from pH studies
J. Biol. Chem.
268
3407-3413
1993
Sus scrofa
Manually annotated by BRENDA team
Van Kuilenburg, A.B.P.; Dobritzsch, D.; Meinsma, R.; Haasjes, J.; Waterham, H.R.; Nowaczyk, M.J.M.; Maropoulos, G.D.; Hein, G.; Kalhoff, H.; Kirk, J.M.; Baaske, H.; Aukett, A.; Duley, J.A.; Ward, K.P.; Lindqvist, Y.; Van Gennip, A.H.
Novel disease-causing mutations in the dihydropyrimidine dehydrogenase gene interpreted by analysis of the three-dimensional protein structure
Biochem. J.
364
157-163
2002
Homo sapiens
Manually annotated by BRENDA team
Schnackerz, K.D.; Dobritzsch, D.; Lindqvist, Y.; Cook, P.F.
Dihydropyrimidine dehydrogenase: a flavoprotein with four iron-sulfur clusters
Biochim. Biophys. Acta
1701
61-74
2004
Cupriavidus necator, Bos taurus, Homo sapiens, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Mattison, L.K.; Johnson, M.R.; Diasio, R.B.
A comparative analysis of translated dihydropyrimidine dehydrogenase cDNA; conservation of functional domains and relevance to genetic polymorphisms
Pharmacogenetics
12
133-144
2002
Mus musculus
Manually annotated by BRENDA team
Di Paolo, A.; Danesi, R.; Ciofi, L.; Vannozzi, F.; Bocci, G.; Lastella, M.; Amatori, F.; Martelloni, B.M.; Ibrahim, T.; Amadori, D.; Falcone, A.; Del Tacca, M.
Improved analysis of 5-Fluorouracil and 5,6-dihydro-5-Fluorouracil by HPLC with diode array detection for determination of cellular dihydropyrimidine dehydrogenase activity and pharmacokinetic profiling
Ther. Drug Monit.
27
362-368
2005
Homo sapiens
Manually annotated by BRENDA team
Van Kuilenburg, A.B.; Meinsma, R.; Beke, E.; Bobba, B.; Boffi, P.; Enns, G.M.; Witt, D.R.; Dobritzsch, D.
Identification of three novel mutations in the dihydropyrimidine dehydrogenase gene associated with altered pre-mRNA splicing or protein function
Biol. Chem.
386
319-324
2005
Sus scrofa, Homo sapiens (Q12882)
Manually annotated by BRENDA team
Ogura, K.; Ohnuma, T.; Minamide, Y.; Mizuno, A.; Nishiyama, T.; Nagashima, S.; Kanamaru, M.; Hiratsuka, A.; Watabe, T.; Uematsu, T.
Dihydropyrimidine dehydrogenase activity in 150 healthy Japanese volunteers and identification of novel mutations
Clin. Cancer Res.
11
5104-5111
2005
Homo sapiens
Manually annotated by BRENDA team
Mattison, L.; Fourie, J.; Hirao, Y.; Koga, T.; Desmond, R.A.; King, J.R.; Shimizu, T.; Diasio, R.B.
The uracil breath test in the assessment of dihydropyrimidine dehydrogenase activity: pharmacokinetic relationship between expired 13CO2 and plasma [2-13C]dihydrouracil
Clin. Cancer Res.
12
549-555
2006
Homo sapiens
Manually annotated by BRENDA team
West, T.P.
Pyrimidine base catabolism in Pseudomonas putida biotype B
Antonie van Leeuwenhoek
80
163-167
2001
Pseudomonas putida
Manually annotated by BRENDA team
van Kuilenburg, A.B.; Meijer, J.; Dobritzsch, D.; Lohkamp, B.; Ruitenbeek, W.; Roelofsen, J.; Abeling, N.G.; Duran, M.; Buzing, C.
Identification of two novel mutations C79X and R235Q in the dihydropyrimidine dehydrogenase gene in a patient presenting with hematuria
Nucleosides Nucleotides Nucleic Acids
27
809-815
2008
Homo sapiens
Manually annotated by BRENDA team
Tsuchida, M.; Yamato, Y.; Hashimoto, T.; Shinohara, H.; Umezu, H.; Yoshiya, K.; Koike, T.; Hayashi, J.
Expression of 5-fluorouracil-related enzymes in lung cancer: ELISA characterizes enzyme activity and messenger RNA expression
Oncol. Rep.
21
1037-1043
2009
Homo sapiens
Manually annotated by BRENDA team
Lohkamp, B.; Voevodskaya, N.; Lindqvist, Y.; Dobritzsch, D.
Insights into the mechanism of dihydropyrimidine dehydrogenase from site-directed mutagenesis targeting the active site loop and redox cofactor coordination
Biochim. Biophys. Acta
1804
2198-2206
2010
Sus scrofa (Q28943)
Manually annotated by BRENDA team
Serve, K.M.; Darnell, J.L.; Takemoto, J.K.; Davies, N.M.; Black, M.E.
Validation of an isocratic HPLC method to detect 2-fluoro-beta-alanine for the analysis of dihydropyrimidine dehydrogenase activity
J. Chromatogr. B
878
1889-1892
2010
Homo sapiens
Manually annotated by BRENDA team
Danielyan, K.E.
Dependence of cell survival on correlative activities of xanthine oxidase and dihydopyrimidine dehydrogenase in human brain-derived cell culture
Cent. Nerv. Syst. Agents Med. Chem.
13
108-113
2013
Homo sapiens
Manually annotated by BRENDA team
Liu, X.Q.; Zhuang, M.; Wang, Z.; Huber, R.M.
Correlation between dihydropyrimidine dehydrogenase and efficacy and toxicity of fluoropyrimidine drugs
Eur. Rev. Med. Pharmacol. Sci.
18
2772-2776
2014
Homo sapiens
Manually annotated by BRENDA team
Camadan, Y.; Oezdemir, H.; Gulcin, I.
Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity
J. Enzyme Inhib. Med. Chem.
31
1335-1341
2016
Ovis aries
Manually annotated by BRENDA team