1.3.1.14: dihydroorotate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.3.1.14

Reaction

(S)-dihydroorotate
+
NAD+
=
Orotate
+
NADH
+
H+

Synonyms

b-type dihydroorotate dehydrogenase, DHO dehydrogenase, DHODase, DHOdehase A, dihydro-orotic dehydrogenase, dihydroorotate dehydrogenase, dihydrorotate dehydrogenase B, L-5,6-dihydro-orotate:NAD oxidoreductase, More, orotate reductase, reductase, orotate

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.14 dihydroorotate dehydrogenase (NAD+)

Storage Stability

Storage Stability on EC 1.3.1.14 - dihydroorotate dehydrogenase (NAD+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18C, prepared enzyme, that is a yellow powder, several weeks, stable
-
-20C, 0.27 M sodium phosphate, pH 6.3, over 6 months, less than 15% loss of activity
-
4C, crystals, over a month, little or no loss of activity
-
4C, holoenzyme, stable for many days
-
above 20C, 0.2 M phosphate buffer, fast loss of activity
-
on ice at room temperature, PyrDI, gradually loses activity over a period of hours
-
room temperature, holoenzyme, stable for many hours
-