1.3.1.14: dihydroorotate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.3.1.14

Reaction

(S)-dihydroorotate
+
NAD+
=
Orotate
+
NADH
+
H+

Synonyms

b-type dihydroorotate dehydrogenase, DHO dehydrogenase, DHODase, DHOdehase A, dihydro-orotic dehydrogenase, dihydroorotate dehydrogenase, dihydrorotate dehydrogenase B, L-5,6-dihydro-orotate:NAD oxidoreductase, More, orotate reductase, reductase, orotate

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.14 dihydroorotate dehydrogenase (NAD+)

Engineering

Engineering on EC 1.3.1.14 - dihydroorotate dehydrogenase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C220A
-
pyrDII mutant, conserved, essential for activity
C225A
-
pyrDII mutant conserved, essential for activity
C228A
-
pyrDII mutant conserved, essential for activity
C230A
-
pyrDII mutant nonconserved
C243A
-
pyrDII mutant conserved, essential for activity
K48A
-
KM-value for dihydroorotate is 2.2fold higher than wild-type value, kcat is 411fold lower than wild-type value
K48E
-
KM-value for dihydroorotate is 4.1fold higher than wild-type value, kcat is 67.5fold lower than wild-type value
K48Q
-
KM-value for dihydroorotate is 1.5fold higher than wild-type value, kcat is 448fold lower than wild-type value
K48R
-
KM-value for dihydroorotate is 2fold lower than wild-type value, kcat is 117fold lower than wild-type value