1.3.1.116: 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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For detailed information about 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase, go to the full flat file.

Reaction

Synonyms

7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase, BaiH

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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Results	in table
1	AA Sequence
4	Cloned(Commentary)
10	Cofactor
3	Expression
6	General Information
26	Inhibitors
4	Metals/Ions
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
21	Organism
2	Pathway
3	pH Optimum
1	pI Value
4	Purification (Commentary)
1	Reaction
9	Reference
3	Specific Activity [micromol/min/mg]
15	Substrates and Products (Substrate)
16	Subunits
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Inhibitors

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Inhibitors on EC 1.3.1.116 - 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,10-phenanthroline

2 entries

Acriflavine

2 entries

Cu2+

[Clostridium] scindens

P32370

1 mM, 1% residual activity

745196

CuCl2

2 entries

EDTA

2 entries

EGTA

[Clostridium] scindens

P32370

-

744377

HgCl2

2 entries

iodoacetate

[Clostridium] scindens

P32370

1.0 mM, 9% inhibition

745196

N-bromosuccinimide

2 entries

N-ethylmaleimide

2 entries

o-phenanthroline

[Clostridium] scindens

P32370

5 mM, 58% residual activity

745196

p-chloromercuriphenyl sulfonate

[Clostridium] scindens

P32370

0.2 mM, 5% residual activity

745196

p-chloromercuriphenyl sulfonic acid

[Clostridium] scindens

P32370

0.2 mM, 95% inhibition

745196

p-chloromercuriphenylsulfonic acid

[Clostridium] scindens

P32370

-

744377

Sodium azide

[Clostridium] scindens

P32370

-

744377

Zn2+

[Clostridium] scindens

P32370

1 mM, 27% residual activity

745196

ZnCl2

2 entries

additional information

[Clostridium] scindens

P32370

iodoacetate and iodoacetamide show no inhibition

744377

-

1,10-phenanthroline

[Clostridium] scindens

P32370

-

744377

1,10-phenanthroline

[Clostridium] scindens

P32370

2.0 mM, 42% inhibition

745196

Acriflavine

[Clostridium] scindens

P32370

-

744377

Acriflavine

[Clostridium] scindens

P32370

0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity

745196

CuCl2

[Clostridium] scindens

P32370

-

744377

CuCl2

[Clostridium] scindens

P32370

1.0 mM, 99% inhibition

745196

EDTA

[Clostridium] scindens

P32370

-

744377

EDTA

[Clostridium] scindens

P32370

5.0 mM, 48% inhibition; 5 mM, 52% residual activity

745196

HgCl2

[Clostridium] scindens

P32370

-

744377

HgCl2

[Clostridium] scindens

P32370

1 mM, 97% inhibition

745196

N-bromosuccinimide

[Clostridium] scindens

P32370

-

744377

N-bromosuccinimide

[Clostridium] scindens

P32370

0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition

745196

N-ethylmaleimide

[Clostridium] scindens

P32370

-

744377

N-ethylmaleimide

[Clostridium] scindens

P32370

1.0 mM, 96% inhibition; 1 mM, 4% residual activity

745196

ZnCl2

[Clostridium] scindens

P32370

-

744377

ZnCl2

[Clostridium] scindens

P32370

1.0 mM, 73% inhibition

745196