1.3.1.111: geranylgeranyl-bacteriochlorophyllide a reductase

This is an abbreviated version!
For detailed information about geranylgeranyl-bacteriochlorophyllide a reductase, go to the full flat file.

Reaction

bacteriochlorophyll a
+ 3 NADP+ =
geranylgeranyl bacteriochlorophyllide a
+ 3 NADPH + 3 H+

Synonyms

BchP, geranylgeranyl-bacteriochlorophyll reductase, geranylgeranyl-bacteriopheophytin reductase, GG-Bphe reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.111 geranylgeranyl-bacteriochlorophyllide a reductase

Cloned

Cloned on EC 1.3.1.111 - geranylgeranyl-bacteriochlorophyllide a reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene bchP, DNA and amino acid sequence determination and analysis, sequence comparison
gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5
gene bchP, recombinant expression in Escherichia coli and in the plasmid-mobilizing Rhodobacter capsulatus strain TecS
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253
gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation