Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.2.7.5: aldehyde ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about aldehyde ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.2.7.5

Reaction

an aldehyde
+
H2O
+
oxidized ferredoxin
=
a carboxylate
+ 2 H+ +
reduced ferredoxin

Synonyms

aldehyde Fd oxidoreductase, aldehyde ferredoxin oxidoreductase, aldehyde oxidase (ferredoxin), aldehyde oxidoreductase, aldehyde: ferredoxin oxidoreductase, aldehyde:ferredoxin oxidoreductase, AOR, Aor1, Aor2, Aor3, Aor4, aorA, AORAa, Athe_0820, Athe_0821, ebA5355, FOR, formaldehyde ferredoxin oxidoreductase, glyceraldehyde 3-phosphate oxidoreductase, Gor, More, oxidase, aldehyde (ferredoxin), PF0346, phenylacetaldehyde:ferredoxin oxidoreductase, POR, Teth514_1380, tungsten-containing aldehyde ferredoxin oxidoreductases, tungsten-containing aldehyde:ferredoxin oxidoreductase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.7 With an iron-sulfur protein as acceptor
                1.2.7.5 aldehyde ferredoxin oxidoreductase

Purification

Purification on EC 1.2.7.5 - aldehyde ferredoxin oxidoreductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
about 120fold, to homogeneity
-
DEAE Sepharose column chromatography and Superdex 200 gel filtration
-
enzyme from vanadium- and molybdenum-grown cells
formaldehyde and aldehyde ferredoxin oxidoreductase
formaldehyde ferredoxin oxidoreductase, 17fold
-
formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required
the enzyme is purified from phenylalanine-grown cells of an overexpressing mutant lacking the gene for an aldehyde dehydrogenase normally involved in anaerobic phenylalanine degradation, purification of the native enzyme by anion exchange chromatography, desalting gel filtration, hydroxyapatite chromatography, ultrafiltration, and gel filtration
to homogeneity under strict anaerobic conditions
-