Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.2.5.1: pyruvate dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about pyruvate dehydrogenase (quinone), go to the full flat file.

Wordmap for 1.2.5.1

Word Map on EC 1.2.5.1 Wordmap for 1.2.5.1

Reaction

Show molfile
pyruvate
+
Show molfile
ubiquinone
+
Show molfile
H2O
=
Show molfile
acetate
+
Show molfile
CO2
+
Show molfile
ubiquinol

Synonyms

EC 1.2.2.2, ECPOX, POX, poxB, POXEC, pqo, pyruvate (quinone) dehydrogenase, pyruvate oxidase, pyruvate oxidase B, pyruvate:quinone oxidoreductase, pyruvate:ubiquinone-8-oxidoreductase, ubiquinione-dependent pyruvate oxidase, ubiquinone-dependent pyruvate oxidase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.5 With a quinone or similar compound as acceptor
                EC 1.2.5.11.2.5.1 pyruvate dehydrogenase (quinone)

Purification

Purification on EC 1.2.5.1 - pyruvate dehydrogenase (quinone)

for references in articles please use BRENDA:EC1.2.5.1

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephadex, ammonium sulfate precipitation, dialysis
-
purified to homogeneity in the absence of detergents
-
the enzyme is purified from an Escherichia coli strain CG3 harboring a plasmid carrying a plasmid th eoxidase gene
-
the poxB4 mutant is purified from the strain CG3, carrying plasmid pYYC16, which overproduces the enzyme about 20fold. Purified on DEAE-cellulose, >90% pure
-