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AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein; AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain
expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth