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1.2.1.8: betaine-aldehyde dehydrogenase

This is an abbreviated version!
For detailed information about betaine-aldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.8

Reaction

Betaine aldehyde
+
NAD+
+
H2O
=
betaine
+
NADH
+ 2 H+

Synonyms

AcBADH, ALD10, aldehyde dehydrogenase, ALDH10, ALDH10A8, ALDH10A9, AMADH, AMADH1, aminoaldehyde dehydrogenase, BADH, BADH1, BADH2, badh2-E2, badh2-E7, betaine aldehyde dehydrogenase, betaine aldehyde dehydrogenase 1, betaine aldehyde dehydrogenase 1, chloroplastic, betaine aldehyde dehydrogenase 2, betaine aldehyde dehydrogenase, chloroplastic, betaine aldehyde oxidase, betaine aldehyde: NAD(P)+ oxidoreductase, betaine aldehyde: NAD+ oxidoreductase, betaine aldehyde:NAD(P)+ oxidoreductase, betaine aldehyde:NAD+ oxidoreductase, betaine-aldehyde dehydrogenase, BetB, dehydrogenase, betaine aldehyde, JcBD1, LcBADH, More, OsALDH10-1, OsALDH10-2, OsBADH1, OsBADH2, PaBADH, PaBADH2, pkBADH, PND BADH2, SACOL2628, YdcW, ZBD1, Zoysia betaine aldehyde dehydrogenase 1

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.8 betaine-aldehyde dehydrogenase

Purification

Purification on EC 1.2.1.8 - betaine-aldehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 enzyme forms: BADH I and BADH II
-
aldehyde dehydrogenase E3 isozyme is a betaine aldehyde dehydrogenase
-
by a two step procedure
For purification of the recombinant protein, cells of 500 ml culture are harvested. Pellet is suspended in lysis buffer and suspension was incubated. Following sonication, suspension is centrifuged. Clear supernatant is collected and loaded onto a Glutathione-Agarose column equilibrated with buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA). The recombinant protein is eluted with elution buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA, and 1.0 mM reduced glutathione). Eluted protein is collected and the purity is analyzed by 12% SDS-PAGE. To remove the GST moiety, the recombinant proteins are digested with PreScission protease.
His-Tag affinity chromatography
Ni Sepharose affinity column chromatography, and gel filtration
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
partial
purified on Q-Sepharose fast flow and 2', 5'-ADP-Sepharose columns, 28 mg of pure protein per liter of culture are yielded
purified to homogeneity following a two-step procedure
-
purified to homogeneity following a two-step procedure, proteins expressed in Escherichia coli
-
rapid purification
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain ER2566 by chitin affinity chromatography
recombinant enzyme from Escherichia coli strain M15 by anion exchange chromatography
Halalkalibacterium halodurans
recombinant His-tagged isozyme AMADH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
the protein undergoes several chromatographic and dialysis steps during purification
-
using nickel-nitrilotriacetic acid agarose affinity chromatography columns after expression in E. coli
using nickel-nitrilotriacetic acid agarose affinity chromatography columns after expression in Escherichia coli
using nickel-nitrilotriacetic acid agarose after cloning and expression in Escherichia coli