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1.2.1.67: vanillin dehydrogenase

This is an abbreviated version!
For detailed information about vanillin dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.67

Reaction

Vanillin
+
NAD+
+
H2O
=
vanillate
+
NADH
+
H+

Synonyms

ALDH, aromatic aldehyde dehydrogenase, CD36-03230, Cd36-03230p, DesV, DesV-like ALDH, LigV, ro02986, SLG_07060, SLG_28320, SN dehydrogenase, syringaldehyde dehydrogenase, vanillin dehydrogenase, VDH, VDH1, VN dehydrogenase, YfmT

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.67 vanillin dehydrogenase

Purification

Purification on EC 1.2.1.67 - vanillin dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography
DEAE-Sepharose column chromatography, Resource Phe column chromatography, and Mono Q column chromatography
-
partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps
purified to apparent electrophoretic homogeneity using His SpinTrap columns
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration