Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.2.1.65: salicylaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about salicylaldehyde dehydrogenase, go to the full flat file.

Wordmap for 1.2.1.65

Word Map on EC 1.2.1.65 Wordmap for 1.2.1.65

Reaction

Show molfile
Salicylaldehyde
+
Show molfile
NAD+
+
Show molfile
H2O
=
Show molfile
salicylate
+
Show molfile
NADH
+ 2 H+

Synonyms

NAD+-dependent salicylaldehyde dehydrogenase, NahF, NahV, SAL dehydrogenase, SALD, SALDan, sALDH, salicylaldehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                EC 1.2.1.651.2.1.65 salicylaldehyde dehydrogenase

Purification

Purification on EC 1.2.1.65 - salicylaldehyde dehydrogenase

for references in articles please use BRENDA:EC1.2.1.65

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni2+ affinity chromatography followed by gel filtration
-
cell free extracts are prepared
-
His-tagged NahF purified by Ni-NTA chromatography
His-tagged NahV purified by Ni-NTA chromatography
native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography