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1.2.1.65: salicylaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about salicylaldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.65

Reaction

Salicylaldehyde
+
NAD+
 +
H2O
=
salicylate
 +
NADH
 + 2 H+

Synonyms

NAD+-dependent salicylaldehyde dehydrogenase, NahF, NahV, SAL dehydrogenase, SALD, SALDan, sALDH, salicylaldehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.65 salicylaldehyde dehydrogenase

Engineering

Engineering on EC 1.2.1.65 - salicylaldehyde dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E175A
site-directed mutagenesis, the apparent Km value of the mutant enzyme to NAD+ is increased by about 2times and the kcat/Km toward the cofactor decreased by 4times. The Km value of the mutant toward salicylaldehyde also increases and the catalytic efficiency decreases by 6times compared to the wild-type enzyme
N149A
site-directed mutagenesis, the catalyic efficiency of the mutant is decreased compared to the wild-type enzyme
V153A
site-directed mutagenesis, the mutation has no significant effect on the kinetic parameters for either NAD+ or salicylaldehyde compared to the wild-type enzyme
additional information