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1.2.1.61: 4-hydroxymuconic-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about 4-hydroxymuconic-semialdehyde dehydrogenase, go to the full flat file.

Reaction

4-hydroxymuconic semialdehyde
+
NAD+
+
H2O
=
maleylacetate
+
NADH
+
H+

Synonyms

4-HS dehydrogenase, 4-hydroxymuconic semialdehyde dehydrogenase, gamma-hydroxymuconic semialdehyde dehydrogenase, hAPE, HqdC, NAD dependent gamma-hydroxymuconic semialdehyde dehydrogenase, PdcG, pnpE

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.61 4-hydroxymuconic-semialdehyde dehydrogenase

Crystallization

Crystallization on EC 1.2.1.61 - 4-hydroxymuconic-semialdehyde dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme PnpE in apo-form or in complex with NAD+, sitting drop vapor diffusion technique, 10 mg/ml apo-enzyme from 20% w/v PEG 3350, and 0.2 M KNO3 at 20°C, 10 mg/ml NAD+-complexed enzyme from 0.1 M bicine, pH 8.5, 20% PEG 10000, and 1 mM NAD+, X-ray diffraction structure determination and analysis at 2.7-3.1 A resolution, molecular replacement method using bovine mitochondrial aldehyde dehydrogenase, PDB ID 1A4Z, as the search model