1.2.1.50: long-chain acyl-protein thioester reductase

This is an abbreviated version!
For detailed information about long-chain acyl-protein thioester reductase, go to the full flat file.

Word Map on EC 1.2.1.50

Reaction

a long-chain aldehyde
+
[protein]-L-cysteine
+
NADP+
=
a [protein]-S-(long-chain fatty acyl)-L-cysteine
+
NADPH
+
H+

Synonyms

acyl coenzyme A reductase, acyl-CoA reductase, AmFAR1, CER4, CpFAS1-R, FACoAR, FAR1, FAR2, FAR3, FAR6, FAR8, fatty acyl-CoA reductase, fatty acyl-CoA reductase 1, fatty acyl-CoA reductase 2, fatty acyl-CoA reductase 3, fatty acyl-CoA reductase 6, fatty acyl-CoA reductase 8, long-chain-fatty-acyl-CoA reductase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.50 long-chain acyl-protein thioester reductase

Cloned

Cloned on EC 1.2.1.50 - long-chain acyl-protein thioester reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of CpFAS1-R domain in a pMAL-c2x vector with maltose binding protein-fusion protein and expression in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
expression in Escherichia coli; overexpression in Escherichia coli
heterologous expression of pYES2.1 plasmid harbouring AmFAR1 in Saccharomyces cerevisiae strain INVSc1
into the vector pET15b for expression in Escherichia coli Rosetta cells; into the vector pET15b for expression in Escherichia coli Rosetta cells; into the vector pET15b for expression in Escherichia coli Rosetta cells; into the vector pET15b for expression in Escherichia coli Rosetta cells; into the vector pET15b for expression in Escherichia coli Rosetta cells
plasmid pHisMBPMarFAR harbouring the sequence of hypothetical protein Maqu_2220 added by His-tag and maltose binding protein transformed to Escherichia coli strain Rosetta (DE3) for heterologous protein expression
-
plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression
-