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1.2.1.5: aldehyde dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about aldehyde dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.2.1.5

Reaction

an aldehyde
+
NAD(P)+
+
H2O
=
a carboxylate
+
NAD(P)H
+
H+

Synonyms

acetaldehyde dehydrogenase, ALD4, aldehyde dehydrogenase, aldehyde dehydrogenase 1A3, aldehyde dehydrogenase 2, aldehyde dehydrogenase 3A1, aldehyde: NAD(P)+ oxidoreductase, ALDH, ALDH1, ALDH13, ALDH17, ALDH1a, ALDH1A1, Aldh1a3, ALDH1b, ALDH2, ALDH2a, ALDH2b, ALDH3, ALDH3A1, Aldh3b1, ALDH3F1, ALDH3H1, ALDH3I1, ALDH7B7, ALDH9, ALDHC, ALDHIII, BcALDH, BCP54, chromosomally encoded box pathway ALDH, class 3 aldehyde dehydrogenase, Corneal 15.8 kDa protein, Corneal protein 54, dehydrogenase, aldehyde (nicotinamide adenine dinucleotide (phosphate)), HTC-ALDH, K-ACDH, MI dehydrogenase, More, myo-inositol dehydrogenase, NAD(P)(+)-dependent aldehyde dehydrogenase, NAD(P)+-dependent aldehyde dehydrogenase, NAD+-dependent Ald4, nicotinprotein aldehyde dehydrogenase, OsALDH2-1, OsALDH2-2, OsALDH2-3, OsALDH2-4, OsALDH2-5, OsALDH22, OsALDH3-1, OsALDH3-2, OsALDH3-3, OsALDH3-4, OsALDH3-5, OsALDH5, OsALDH7, PEG-ALDH, PuuC, ST0064, succinic semialdehyde dehydrogenase, Transparentin, Tumor-associated aldehyde dehydrogenase, ybcD

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.5 aldehyde dehydrogenase [NAD(P)+]

Purification

Purification on EC 1.2.1.5 - aldehyde dehydrogenase [NAD(P)+]

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Phenyl-Toyopearl, Red-Sepharose
DEAE MacroPrep column chromatography
DEAE-cellulose column chromatography, 5’-AMP Sepharose 4B and 4-hydroxyacetophenone affinity chromatography
-
immobilized metal ion affinity column chromatography
isoenzyme F1 and F2
-
Ni-affinity chromatography
-
Ni2+-NTA resin column chromatography
-
nickel affinity column chromatography
on a Ni-NTA column
-
partial
-
purification of the enzyme(s) responsible for the GSH/DHLA-dependent NAD+ reduction activity from large-scale cell cultures, native enzyme from EKVX lung cancer cells by ultracentrifugation of crude cell extract and anion echange chromatography, hydrophobic interaction and hydroxyapatite chromatography, followed by gel filtration and another different step of anion exchange chromatography
recombinant tagged enzyme from Escherichia coli strain ER2566 by metal chelating affinity chromatography, desalting gel filtration, and dialysis
Sephacryl S-300 gel filtration
single-step purification
to homogeneity by nickel affinity chromatography and gel filtration