1.2.1.48: long-chain-aldehyde dehydrogenase

This is an abbreviated version!
For detailed information about long-chain-aldehyde dehydrogenase, go to the full flat file.

Reaction

Synonyms

ALDH, ALDH10, ALDH3A2, Aldh3b1, ALDH3B2, ALDH3B3, Bt-Aldh, dehydrogenase, long-chain aliphatic aldehyde, FAldDH, FALDH, fatty aldehyde dehydrogenase, fatty aldehyde:NAD+ oxidoreductase, long-chain aldehyde dehydrogenase, long-chain fatty aldehyde dehydrogenase, long-chain-aldehyde dehydrogenase, membrane-bound fatty aldehyde dehydrogenase

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.48 long-chain-aldehyde dehydrogenase

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Results	in table
3	Activating Compound
89	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
24	Cofactor
4	Crystallization (Commentary)
273	Disease/ Diagnostics
2	Expression
11	General Information
28	Inhibitors
34	KM Value [mM]
32	Localization
10	Metals/Ions
15	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
32	Organism
1	Pathway
6	pH Optimum
1	pH Range
3	Posttranslational Modification
19	Protein Variants
8	Purification (Commentary)
2	Reaction
5	Reaction Type
30	Reference
28	Source Tissue
18	Specific Activity [micromol/min/mg]
4	Storage Stability
137	Substrates and Products (Substrate)
17	Subunits
37	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.48 - long-chain-aldehyde dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0

Geobacillus thermoleovorans

Q9FAB1

697840

including chromatography in a Resource Q column, recombinant enzyme from Escherichia coli also purified

Acinetobacter sp.

-

390325

metal affinity column chromatography

Marinobacter nauticus

-

762634

Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration

Staphylococcus aureus

-

762884

recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity

Homo sapiens

-

693389

recombinant Strep-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration

Homo sapiens

P51648

743336

Strep-tag affinity chromatography and AMP-Sepharose column chromatography

Rattus norvegicus

-

725656

using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B

Homo sapiens

-

390324