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1.2.1.47: 4-trimethylammoniobutyraldehyde dehydrogenase

This is an abbreviated version!
For detailed information about 4-trimethylammoniobutyraldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.47

Reaction

4-Trimethylammoniobutanal
+
NAD+
+
H2O
=
4-Trimethylammoniobutanoate
+
NADH
+ 2 H+

Synonyms

4-N-trimethylaminobutyraldehyde dehydrogenase, 4-N-trimethylaminobutyraldehyde dehydrogenase II, 4-trimethylaminobutyraldehyde dehydrogenase, 4-trimethylammoniobutyraldehyde dehydrogenase, aldehyde dehydrogenase 9A1, Aldh9a1, carnitine biosynthesis enzyme, dehydrogenase, trimethylaminobutyraldehyde, gamma-trimethylaminobutyraldehyde dehydrogenase, TMABA dehydrogenase, TMABA-DH, TMABADH, TMABAL dehydrogenase, TMABaldehyde-DH II, TMABALDH

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.47 4-trimethylammoniobutyraldehyde dehydrogenase

Purification

Purification on EC 1.2.1.47 - 4-trimethylammoniobutyraldehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
14.2fold to apparent homogeneity, by hydrophobic chromatography and affinity chromatography
-
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography
recombinant FLAG-tagged enzyme TMABA-DH from HEK-293T cells by immunoaffinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) pLysS by cobalt affinity and nickel affinity chromatography, followed by ultrafiltration and gel filtration
recombinant SUMO-His6-tagged ALDH9A1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the SUMO-His tag, dialysis, ultrafiltration, and gel filtration
using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose
-
using column chromatography on SP-Sepharose, Red Sepharose CL-6B and Hydroxylapatite