1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
This is an abbreviated version!
For detailed information about methylmalonate-semialdehyde dehydrogenase (CoA-acylating), go to the full flat file.
Word Map on EC 1.2.1.27
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1.2.1.27
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lysosomotropic
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3-hydroxyisobutyrate
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coa-dependent
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mississippi
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propionyl-coa
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aldh6a1
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3-hydroxypropionic
- 1.2.1.27
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lysosomotropic
- 3-hydroxyisobutyrate
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coa-dependent
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mississippi
- propionyl-coa
- aldh6a1
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3-hydroxypropionic
Reaction
Synonyms
Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MGG_01606, MMSADH, MMSDH, MoMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218
ECTree
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Purification
Purification on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
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MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity
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recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3). Among the twelve molecules in the asymmetric unit, six subunits complexed with NAD+
to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration
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MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin