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1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

This is an abbreviated version!
For detailed information about methylmalonate-semialdehyde dehydrogenase (CoA-acylating), go to the full flat file.

Word Map on EC 1.2.1.27

Reaction

2-Methyl-3-oxopropanoate
+
CoA
 +
H2O
 +
NAD+
=
propanoyl-CoA
 +
HCO3-
 +
NADH

Synonyms

Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MGG_01606, MMSADH, MMSDH, MoMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

Cloned

Cloned on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene dddC, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene FG99_15390, recombinant expression of the codon-optimized, His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene MGG_01606, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR expression analysis
in Escherichia coli DH5[alpha] transformants containing the pSKmsdbsub plasmid
into the pGEM-T easy vector for sequencing
SSO1218 expressed in Escherichia coli BL21 (DE3) RIL via isopropyl-b-D-thiogalactopyranoside induction