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1.2.1.23: 2-oxoaldehyde dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about 2-oxoaldehyde dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.2.1.23

Reaction

a 2-oxoaldehyde
+
NAD+
+
H2O
=
a 2-oxo carboxylate
+
NADH
+
H+

Synonyms

2-ketoaldehyde dehydrogenase, ADH1, alpha-ketoaldehyde dehydrogenase, methylglyoxal dehydrogenase, MGD, NAD+-linked alcohol dehydrogenase 1, NAD+-linked methylglyoxal dehydrogenase, NAD-dependent alpha-ketoaldehyde dehydrogenase, NAD-linked alpha-ketoaldehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.23 2-oxoaldehyde dehydrogenase (NAD+)

Purification

Purification on EC 1.2.1.23 - 2-oxoaldehyde dehydrogenase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200
-
using acetone powder extract, ammonium sulfate precipitation, calcium phosphate gel treatment, column chromatography on DEAE-cellulose and NAD-Agarose, separated from the NADP-dependent enzyme through affinity chromatography on a thiol-Sepharose column
-
using acetone powder preparation, ammonium sulfate, column chromatography on DEAE-cellulose, affinity column packed with TSK-Gel AF-Red Toyopearl 650 ML and fast protein liquid chromatography
-
using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, phosphocellulose, hydroxyapatite and Sephadex G-200
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Phosphocellulose, Sephadex G-200, Hydroxylapatite, and a second DEAE-cellulose step
-
using column chromatography on DEAE-cellulose, Butyl-Toyopearl 650M, Sephadex G-150, hydroxylapatite, Blue-dextran and Sepharose CL-6B
-
using treatment with acetone, ammonium sulfate fractionation, and gel filtration on Sephadex G-25
-