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1.2.1.16: succinate-semialdehyde dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about succinate-semialdehyde dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.2.1.16

Reaction

succinate semialdehyde
+
NAD(P)+
 +
H2O
=
succinate
 +
NAD(P)H
 +
H+

Synonyms

3-sulfopropanal dehydrogenase, ALDH, BADH, BsSSADH, cce4228, cce4228 protein, dehydrogenase, succinate semialdehyde (nicotinamide adenine dinucleotide (phosphate)), gabD, GabD dehydrogenase, GabD1, gapN-2, More, NAD(P)+-coupled 3-sulfopropanal dehydrogenase, NAD(P)+-dependent benzaldehyde dehydrogenase, NAD(P)+-dependent succinate semialdehyde dehydrogenase, NAD(P)+-dependent succinic semialdehyde dehydrogenase, NAD(P)-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent benzaldehyde dehydrogenase, NAD+/NADP+-dependent SSADH, SpSSADH, SSA dehydrogenase, SSADH, SSADH-II, SSADHase, SSDH, SSO1842, ST0064, succinate semialdehyde dehydrogenase, succinate semialdehyde dehydrogenase (nicotinamide adenine dinucleotide (phosphate)), succinate-semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase (NAD(P)), succinate-semialdehyde dehydrogenase (NAD(P)+), succinic semialdehyde dehydrogenase, succinic semialdehyde:NAD(P)+ oxidoreductase, sulfopropanal dehydrogenase, YneI

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.16 succinate-semialdehyde dehydrogenase [NAD(P)+]

Cloned

Cloned on EC 1.2.1.16 - succinate-semialdehyde dehydrogenase [NAD(P)+]

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 (DE3)-RIL cells
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells
-
expressed in Escherichia coli strain BL21(DE3)pLysS
-
expression in Escherichia coli
gabD gene cloned, expression in Escherichia coli
gene gabD or cce4228, DNA and amino acid sequence deternination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene gapD, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SSADH, quantitative real-time PCR enzyme expression analysis
gene ssadh, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpressed as His-tagged GabD
-
Protein expression in Escherichia coli strain JM109. Single colonies are picked and the DNA is isolated and screened for the desired mutation either by restriction analysis (C103A and C249A) or by sequencing (C140A and C220A). The overall fidelity of the PCR amplification and the presence of the desired mutations are confirmed by sequencing. The plasmids containing the mutated genes are then transformed into Escherichia coli strain BL21(DE3)pLysS for protein expression.
-
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
-