1.2.1.15: malonate-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about malonate-semialdehyde dehydrogenase, go to the full flat file.

Reaction

3-oxopropanoate
+
NAD(P)+
+
H2O
=
malonate
+
NAD(P)H
+ 2 H+

Synonyms

dehydrogenase, malonate semialdehyde, IolA, KES23460, NAD-dependent malonate-semialdehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.15 malonate-semialdehyde dehydrogenase

Crystallization

Crystallization on EC 1.2.1.15 - malonate-semialdehyde dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8°C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model