1.2.1.15: malonate-semialdehyde dehydrogenase

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For detailed information about malonate-semialdehyde dehydrogenase, go to the full flat file.

Reaction

Synonyms

dehydrogenase, malonate semialdehyde, IolA, KES23460, NAD-dependent malonate-semialdehyde dehydrogenase

ECTree

1 Oxidoreductases

1.2 Acting on the aldehyde or oxo group of donors

1.2.1 With NAD⁺ or NADP⁺ as acceptor

1.2.1.15 malonate-semialdehyde dehydrogenase

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1	Application
1	CAS Registry Number
2	Cloned(Commentary)
3	Cofactor
1	Crystallization (Commentary)
2	General Information
2	General Stability
10	Inhibitors
3	Ki Value [mM]
2	KM Value [mM]
3	Metals/Ions
1	Natural Substrates/ Products (Substrates)
6	Organism
3	Pathway
1	pH Optimum
1	Protein Variants
2	Purification (Commentary)
1	Reaction
3	Reaction Type
4	Reference
1	Specific Activity [micromol/min/mg]
2	Storage Stability
4	Substrates and Products (Substrate)
2	Subunits
4	Synonyms
1	Systematic Name
1	Temperature Stability [°C]

Crystallization

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Crystallization on EC 1.2.1.15 - malonate-semialdehyde dehydrogenase

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purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8°C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model

Pseudomonas sp.

741539