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1.2.1.11: aspartate-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about aspartate-semialdehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.11

Reaction

L-aspartate 4-semialdehyde
+
phosphate
 +
NADP+
=
L-4-aspartyl phosphate
 +
NADPH
 +
H+

Synonyms

AFUA_3G06830, ASA dehydrogenase, ASA DH, ASA-DH, ASADH, ASADHD, Asd, ASD enzyme, Asd1, Asd2, AsdA, aspartate beta-semialdehyde dehydrogenase, aspartate semialdehyde dehydrogenase, aspartate-beta-semialdehyde dehydrogenase, aspartate-beta-semialdeyhyde dehydrogenase, aspartate-semialdehyde dehydrogenase, aspartic acid semialdehyde dehydrogenase, aspartic beta-semialdehyde dehydrogenase, aspartic semialdehyde dehydrogenase, aspartyl beta-semialdehyde dehydrogenase, aspartyl semialdehyde dehydrogenase, BDCG_01946, CNA02450, dehydrogenase, aspartate semialdehyde, ecASADH, FtASADH, hiASADH, L-aspartate-beta-semialdehyde dehydrogenase, L-aspartate-beta-semialdehyde:NADP oxidoreductase (phosphorylating), Rv3708c

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.11 aspartate-semialdehyde dehydrogenase

Purification

Purification on EC 1.2.1.11 - aspartate-semialdehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion exchange chromatography and gel-filtration
-
by using affinity chromatography and gel filtration
cobalt immobilized metal-affinity column chromatography and Source 30Q column chromatography
K-12, using ammonium sulfate precipitation and column chromatography on Sephadex G-200 and DEAE-Sephadex A-50
-
Ni-affinity chromatography
-
Ni-affinity chromatography and anion exchange chromatography
-
Ni-NTA column chromatography and gel filtration
partially, recombinant enzyme from Escherichia coli, 37fold
recombinant C-terminally His-tagged enzyme ASADH from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and dialysis
recombinant C-terminally His-tagged enzyme ASADH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant enzyme from Escherichia coli to over 95% purity by anion exchange chromatography and gel filtration
-
recombinant enzyme from strain BL21(DE3) in several steps
recombinant His-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography, followed by ultrafiltration
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to 96% purity
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type and mutants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
to homogeneity
using a Co2+ affinity matrix and anion exchange chromatography
-
using ammonium sulfate precipitation and column chromatography on Q Sepharose XL and Procion Red-A
-
using ammonium sulfate precipitation and column chromatography on Sephadex G-200, DEAE-cellulose and hydroxylapatite
-
using anion-exchange chromatography, Source 15Q, followed by gel filtration
-
using anionic exchange chromatography on DEAE EMD 650 column and Q-Sepharose column
using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, hydroxylapatite, ultrogel ACA-44 and Sepharose C3
-
using column chromatography on Q Sepharose XL and omega-aminohexyl-agarose for the purification of V. cholerae I and column chromatography on Q Sepharose XL for the purification of V. cholerae II
-
using column chromatography on Q Sepharose XL and phenyl-Sepharose
using heat treatment, acid precipitation, protamine sulfate treatment and ammonium sulfate precipitation
-
using heat treatment, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-200
-
using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200
-