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1.2.1.105: 2-oxoglutarate dehydrogenase system

This is an abbreviated version!
For detailed information about 2-oxoglutarate dehydrogenase system, go to the full flat file.

Word Map on EC 1.2.1.105

Reaction

2-oxoglutarate
+
CoA
+
NAD+
=
succinyl-CoA
+
CO2
+
NADH

Synonyms

2-OGDH2, 2-oxoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase complex, alpha-KDE2, alpha-ketoglutarate dehydrogenase, alpha-ketoglutarate dehydrogenase complex, alpha-KGDH, At3g55410, At5g65750, DHTKD1, dihydrolipoyl succinyltransferase E2, E1a, E1k, E1o, E2, KGDH, KGDHC, More, MPA24.10, ODGH, ODGH1, ODGH2, ODH, OGDC, OGDH, OGDHC, OGDHL, OGHDC-E2, PDHC, SucA, SucB

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.105 2-oxoglutarate dehydrogenase system

Purification

Purification on EC 1.2.1.105 - 2-oxoglutarate dehydrogenase system

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2-oxoglutarate dehydrogenase complex
2-oxoglutarate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration
-
by gel filtration
-
dissociation of the 2-oxoglutarate dehydrogenase complex into two fractions on a calcium phosphate gel-cellulose column in the presence of 2.5 M urea and 1% ammonium sulfate releases lipoamide dehydrogenase. The second fraction precipitates with between 0.23 and 0.40 saturation of ammonium sulfate and exhibits 2-oxoglutarate dehydrogenase and lipoate succinyltransferase activities. All three of the isolated enzymes are required to catalyze a CoA and NAD-linked oxidation of 2-oxoglutarate
-
partially purified from skeletal muscle of euthermic and hibernating ground squirrels
-
purification procedure for the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes from mitochondria. After fractionated precipitations with polyethylene glycol, elimination of thiol proteins, and gel-filtration chromatography, the resulting preparations contain both activities. Covalent chromatography on thiol-activated Sepharose CL-4B allows the specific binding of the 2-oxoglutarate dehydrogenase complex activity in the presence of 2-oxoglutarate, the pyruvate dehydrogenase complex activity is retained in the presence of pyruvate
-