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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
no reconstitution of whole complex, pyruvate dehydrogenase subunit regains some activity after separation from complex in the presence of chaperonins and ATP/MgCl2
treatment of the E2-X subcomplex with 4 M guanidinium chloride causes a complete loss of enzymatic activity and the dissociation of the subcomplex into monomeric 1.5-3 S species. Removal of the chaotrope by dialysis for 18 h results in complete restoration of E2 enzymatic activity and reassembly of a 32 S subcomplex