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1.2.1.104: pyruvate dehydrogenase system

This is an abbreviated version!
For detailed information about pyruvate dehydrogenase system, go to the full flat file.

Reaction

pyruvate
+
CoA
 +
NAD+
=
acetyl-CoA
 +
CO2
 +
NADH

Synonyms

aceE, AceF, At1g54220, At3g13930, At3g52200, CTHT_0006350, CTHT_0069820, dihydrolipoamide acetyltransferase, dihydrolipoyllysine-residue acetyltransferase component, DLAT, DLD, DLST, E1 component of pyruvate dehydrogenase complex, E1 component subunit alpha, E1 component subunit beta, IAR4, LpdA, Lta3, MAB1, More, Mrp-3, Pda1, Pda1p, PDC, PDCp, PDH, PDH complex, PDH-E1 catalytic subunit, PDHa, PdhA1, PDHalpha, PdhB1, PDHC, PdhE, PDHE1alpha1, PdhH, PdhX, PH2, plastidial pyruvate dehydrogenase complex, pyruvate decarboxylase, pyruvate dehydrogenase, pyruvate dehydrogenase alpha subunit, pyruvate dehydrogenase complex, pyruvate dehydrogenase E1

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.104 pyruvate dehydrogenase system

Purification

Purification on EC 1.2.1.104 - pyruvate dehydrogenase system

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha and beta subunits purified from enzyme complex after gel filtration in the presence of 2 M KI followed by chromatography on hydroxyapatite in the presence of 8 M urea
-
isolation of EC 1.2.4.1 after elastase treatment of the complex and gel filtration
-
Ni2+-Sepharose column chromatography
-
purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1
-
purification procedure for the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes from mitochondria. After fractionated precipitations with polyethylene glycol, elimination of thiol proteins, and gel-filtration chromatography, the resulting preparations contain both activities. Covalent chromatography on thiol-activated Sepharose CL-4B allows the specific binding of the 2-oxoglutarate dehydrogenase complex activity in the presence of 2-oxoglutarate, the pyruvate dehydrogenase complex activity is retained in the presence of pyruvate
-
purification scheme for the pyruvate dehydrogenase complex directly from body wall muscle which yields a fully activated pyruvate dehydrogenase complex with substantial PDHa kinase activity
-
pyruvate dehydrogenase can be resolved from complex by incubation with 8 M urea, 4 M guanidinium chloride, 100 mM glycine/NaOH, pH 9.0 followed by gel filtration
-
pyruvate dehydrogenase complex composed of EC 1.2. 4.1, EC 2.3.1.12, EC 1.8.1.4
Pigeon
-
pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4
pyruvate dehydrogenase complex from ox heart, by PEG fractionation, ultracentrifugation, and gel filtration
-
pyruvate dehydrogenase complex, composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4
pyruvate dehydrogenase purified from complex by gel filtration
-
recombinant multienzyme complex components from Escherichia coli strain BL21(DE3) to homogeneity
-
The enzyme complex is resolved into its constituent proteins by means of gel filtration on Sepharose CL-6B in the presence of 2 M-KI, followed by chromatography on hydroxyapatite in the presence of 8 M urea. These harsh conditions are necessary to cause suitable dissociation of the enzyme complex
-