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1000000
and 2000000, gel filtration, enzyme isolated from mitochondria-enriched high sedimentation rate fraction
182000 - 183000
-
light scattering
190000
-
sedimentation equilibrium centrifugation
206000
-
scanning transmission electron microscopy
230000
gel filtration, mammalian E1p, mE1palpha (PDHA1) and mE1pbeta (PDHB1), and mE3 components are detected mostly in sub-megadalton size fractions of the high ionic strength fraction
230000 - 440000
gel filtration, enzyme isolated from nuclei-enriched low sedimentation rate fraction
40000
-
alpha2,beta2, 2 * 43000 + 2 * 40000, SDS-PAGE, immunoblotting
50000
-
60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex
52000
-
60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex
55000
-
60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex
56000
-
alpha2,beta2, 2 * 38000 + 2 * 56000, SDS-PAGE, N-terminal amino acid sequencing
92500
-
x * 92500, SDS-PAGE
94000
-
2 * 94000, SDS-PAGE
99474
-
2 * 99474, calculation from nucleotide sequence
100000
-
sucrose density gradient centrifugation
100000
-
1 * 100000, SDS-PAGE
100000
-
x * 100000, SDS-PAGE
100000
-
2 * 100000, SDS-PAGE
2000000
and 1000000, gel filtration, enzyme isolated from mitochondria-enriched high sedimentation rate fraction
2000000
gel filtration, E2 component eluted only as a megadalton complex even in the high sedimentation rate fraction
35000
-
alpha2,beta2, 2 * 45000 + 2 * 35000, SDS-PAGE
35000
-
alpha2,beta2, 2 * 45000 + 2 * 35000, SDS-PAGE
35000
-
alpha2,beta2, 2 * 41000 + 2 * 35000, SDS-PAGE
36000
-
alpha2,beta2, 2 * 41000 + 2 * 36000
36000
-
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence
36000
-
60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex
37000
Pigeon
-
alpha2,beta2, 2 * 42000 + 2 * 37000, SDS-PAGE
37000
alpha2,beta2, 1 * 43000 + 1 * 41000 + 2 * 37000, SDS-PAGE
37000
-
x * 40000-41000 + x * 37000
38000
-
alpha2,beta2, 2 * 42000 + 2 * 38000, SDS-PAGE
38000
-
alpha2,beta2, 2 * 38000 + 2 * 56000, SDS-PAGE, N-terminal amino acid sequencing
41000
-
alpha2,beta2, 2 * 41000 + 2 * 36000
41000
alpha2,beta2, 1 * 43000 + 1 * 41000 + 2 * 37000, SDS-PAGE
41000
-
alpha2,beta2, 2 * 41000 + 2 * 35000, SDS-PAGE
41000
-
60 * 41000 (E1 alpha) + 60 * 36000 (E1 beta) + 60 * 52000 (E2, EC 2.3.1.12) + 12 * 55000 (E3, EC 1.8.1.4) + 8-12 * 50000 (component X) + kinase and phosphatase components, heart pyruvate dehydrogenase complex
42000
-
alpha2,beta2, 2 * 42000 + 2 * 38000, SDS-PAGE
42000
Pigeon
-
alpha2,beta2, 2 * 42000 + 2 * 37000, SDS-PAGE
42000
-
alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence
43000
alpha2,beta2, 1 * 43000 + 1 * 41000 + 2 * 37000, SDS-PAGE
43000
-
alpha2,beta2, 2 * 43000 + 2 * 40000, SDS-PAGE, immunoblotting
45000
-
alpha2,beta2, 2 * 45000 + 2 * 35000, SDS-PAGE
45000
-
alpha2,beta2, 2 * 45000 + 2 * 35000, SDS-PAGE
additional information
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additional information
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additional information
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MW of native complex from heart: 740000 Da
additional information
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MW of native complex isolated from kidney and heart: 7000000 Da and 8500000 Da respectively