1.2.1.100: 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

This is an abbreviated version!
For detailed information about 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, go to the full flat file.

Reaction

5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
+
NAD+
+
H2O
=
3-hydroxy-2-methylpyridine-4,5-dicarboxylate
+
NADH
+
H+

Synonyms

mlr6739, mlr6793

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

Natural Substrates Products

Natural Substrates Products on EC 1.2.1.100 - 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O
3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
show the reaction diagram
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additional information
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enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+ and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH, i.e. reaction of 4-pyridoxic acid dehydrogenase. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate
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