1.2.1.100: 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

This is an abbreviated version!
For detailed information about 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, go to the full flat file.

Reaction

5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
+
NAD+
+
H2O
=
3-hydroxy-2-methylpyridine-4,5-dicarboxylate
+
NADH
+
H+

Synonyms

mlr6739, mlr6793

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.100 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

Engineering

Engineering on EC 1.2.1.100 - 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E149Q
mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH
H137L
almost complete loss of activity
E149Q
-
mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH
-
H137L
-
almost complete loss of activity
-