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1.14.20.4: anthocyanidin synthase

This is an abbreviated version!
For detailed information about anthocyanidin synthase, go to the full flat file.

Word Map on EC 1.14.20.4

Reaction

a (2R,3S,4S)-leucoanthocyanidin
+
2-oxoglutarate
 +
O2
=
a (4S)- 2,3-dehydroflavan-3,4-diol
 +
succinate
 +
CO2
 +
H2O

Synonyms

ANS, ANS-1, ANS-2, anthocyanidin synthase, anthocyanidin synthase 1, anthocyanidine synthase, anthocyanin synthase, FcANS1, GbANS, IbANS, LDOX, leucoanthocyanidin dioxygenase, leucoanthocyanidin oxygenase, leucocyanidin dioxygenase, leucocyanidin oxygenase, leucocyanidin,2-oxoglutarate:oxygen oxidoreductase, McANS-1, McANS-2, MdANS, RtLDOX

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.4 anthocyanidin synthase

Cloned

Cloned on EC 1.14.20.4 - anthocyanidin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a full-length, 1427-bp long cDNA named RnANS1 (Ribes nigrum anthocyanidin synthase 1), which is homologous to the anthocyanidin synthase gene, is cloned from blackcurrant using a homologous cloning strategy. RnANS1 is highly homologous to other plant ANS genes at both the nucleotide and amino acid sequence levels. The deduced protein contains domains conserved in the 2-oxoglutarate and Fe(II)-dependent oxygenase, and is phylogenetically closely related to Paeonia suffruticosa and Paeonia lactiflora
analysis of expression in wheat-rye hybrids
anthocyanin synthase is cloned from Magnolia sprengeri (MsANS) using rapid amplification of complementary DNA ends technology. The full-length MsANS is 1171-bp long and contains a 1080-bp open reading frame encoding a 360 amino acid polypeptide. In a sequence alignment analysis, the deduced MsANS protein showed high identity to ANS proteins from other plants: Prunus salicina var. cordata (74% identity), Ampelopsis grossedentata (74% identity), Pyrus communis (73% identity), and Prunus avium (73% identity)
expressed in Escherichia coli
expressed in Escherichia coli strain DH5alpha
-
expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli, overexpression in rice mutant Nootripathu, which accumulates proanthocyanidins exclusively in pericarp
expression in Saccharomyces cerevisiae
gene BjANS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
overexpression in Escherichia coli
the full-length cDNA of Reaumuria trigyna leucoanthocyanidin dioxygenase (RtLDOX) is cloned into the expression vector pET32a and expressed in Escherichia coli Rosetta (DE3). The RtLDOX recombinant protein is able to replace flavanone-3-hydroxylase (EC 1.14.11.9), another dioxygenase in the flavonoid pathway, to convert naringenin to dihydrokaempferol in vitro. the enzyme can complement the Arabidopsis LDOX mutant transparent testa11 (tt11-11), which has reduced proanthocyanin and anthocyanin levels in seeds, to accumulate these two compounds