1.14.19.9: tryptophan 7-halogenase

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For detailed information about tryptophan 7-halogenase, go to the full flat file.

Word Map on EC 1.14.19.9

1.14.19.9

fadh2-dependent

dichlorination

halometabolites

rebeccamycin

alpha-ketoglutarate-dependent

haloperoxidases

syringomycin

alphakg-dependent

pyrrolnitrin

alphakg

l-thr

organohalogens

Reaction

+ 2 H2O

Synonyms

EC 1.14.14.7, FAD-dependent halogenase, flavin-dependent halogenase, flavin-dependent tryptophan halogenase, halogenase, KtzQ, L-tryptophan-7-halogenase, PrnA, RebH, Trp 7-hal, Trp halogenase, Trp-7-Hal, Trp-7-halogenase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.19.9 tryptophan 7-halogenase

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Results	in table
578	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
15	Cofactor
5	Crystallization (Commentary)
3	General Information
1	Inhibitors
9	KM Value [mM]
3	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
23	Organism
5	Pathway
9	PDB ID
4	pH Optimum
10	Protein Variants
8	Purification (Commentary)
8	Reaction
7	Reaction Type
23	Reference
3	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
2	Subunits
32	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
11	Turnover Number [1/s]

Purification

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Purification on EC 1.14.19.9 - tryptophan 7-halogenase

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2 entries

nickel chelating Sepharose column chromatography

Pseudomonas fluorescens

726682

RebH is purified by size-exclusion chromatography

Lentzea aerocolonigenes

672189

recombinant enzyme

Lentzea aerocolonigenes

Q8KHZ8

676785, 695031

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography

Lentzea aerocolonigenes

Q8KHZ8

744712

recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography and gel filtration

Kutzneria sp. 744

A8CF75

745152