1.14.19.9: tryptophan 7-halogenase

This is an abbreviated version!
For detailed information about tryptophan 7-halogenase, go to the full flat file.

Word Map on EC 1.14.19.9

Reaction

tryptophan
+
FADH2
+
chloride
+
O2
+
H+
=
7-chloro-L-tryptophan
+
FAD
+ 2 H2O

Synonyms

FAD-dependent halogenase, flavin-dependent halogenase, flavin-dependent tryptophan halogenase, halogenase, KtzQ, L-tryptophan-7-halogenase, PrnA, RebH, Trp 7-hal, Trp halogenase, Trp-7-Hal, Trp-7-halogenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.9 tryptophan 7-halogenase

Purification

Purification on EC 1.14.19.9 - tryptophan 7-halogenase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
nickel chelating Sepharose column chromatography
-
RebH is purified by size-exclusion chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography
-
recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography and gel filtration