1.14.19.3: acyl-CoA 6-desaturase

This is an abbreviated version!
For detailed information about acyl-CoA 6-desaturase, go to the full flat file.

Word Map on EC 1.14.19.3

Reaction

alpha-linolenoyl-CoA
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
stearidonoyl-CoA
+ 2 ferricytochrome b5 + 2 H2O

Synonyms

acyl-CoA DELTA6-desaturase, acyl-CoA desaturase, acyl-CoA-dependent DELTA6-desaturase, AsD6DES, D6 desaturase, d6-des, D6D, D6Des, DEALTA6I, delta 6 desaturase, DELTA 6-desaturase, delta 6-fatty acid desaturase, DELTA-6 desaturase, delta-6 fatty acid desaturase, DELTA-6-desaturase, DELTA6 desaturase, DELTA6 fatty acid desaturase, DELTA6 fatty acyl desaturases, DELTA6-acyl CoA desaturase, DELTA6-acyl-group desaturase, DELTA6-desaturase, DELTA6-desaturase II, DELTA6-fatty acid desaturase, DELTA6-fatty acyl-CoA desaturase, delta61, DELTA6fad_b, DES6, desaturase, fatty acid DA6-, desaturase, linoleate, DesI, EaD6DES, EC 1.14.99.25, FA desaturase 2, FADS2, FADS6, fadsd6, FAT-3, fatty acid 6-desaturase, fatty acid DA6-desaturase, fatty acid DELTA 6-desaturase, fatty acid delta-6 desaturase, fatty acid DELTA6-desaturase, fatty acid desaturase 2, fatty acid desaturase-2, GcFADS2, linoleate desaturase, linoleic acid desaturase, linoleic desaturase, linoleoyl CoA desaturase, linoleoyl-coenzyme A desaturase, long-chain fatty acid DELTA6-desaturase, MaFADS6, MaFADS6-I, Md6, MpFADS6, PiDesD6, TpFADS2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.3 acyl-CoA 6-desaturase

Cloned

Cloned on EC 1.14.19.3 - acyl-CoA 6-desaturase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
D6D, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis; D6D-V, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
B1PJR8, B2ZE91
DELTA6-desaturase gene identified in human chromosome 11
-
DELTA6-desaturase II cDNA expressed in Aspergillus oryzae
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional expression in Saccharomyces cerevisiae strain INVSc1
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expressed in Camelina sativa
expressed in Escherichia coli DH5alpha, co-expressed with cytochrome b5 and ferredoxin, no enzyme activity when expressed in Escherichia coli alone
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expressed in HEK-293 cells
expressed in Pichia pastoris GS115; phylogenetic tree, expression in Pichia pastoris GS115, molecular mechanisms underlying the elevated expression of fatty acid desaturases induced by a decrease in the environmental temperature
expressed in Pichia pastoris strain GS115
expressed in Saccharomyces cerevisiae
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expressed in Saccharomyces cerevisiae strain BY4741, two different mRNA variants
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expressed in Saccharomyces cerevisiae strain InvSc1
expressed in Saccharomyces cerevisiae strain InvSc1; expression in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae strain INVScl
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expressed in Saccharomyces cerevisiae, strain DBY746 with and without supplementation with linoleic acid
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expression in MCF7 cells
expression in Saccharomyces cerevisaiae
expression in Saccharomyces cerevisiae
expression of N-terminal truncated enzyme in Escherichia coli
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expression of wild-type, A181T/A188G/Y189F/S205N/L206T/G207A, A188G/Y189F, A181T/A200F and A181T/A200F/S205N/L206T/G207A mutant enzyme in Escherichia coli
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gene D6, recombinant expression in Saccharomyces
gene d6-des, DNA and amino acid sequence determination and analysis, sequence comparisons
gene D6DES, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional expression in Saccharomyces cerevisiae strain INVSc1 producing gamma-linolenic acid and stearidonic acid at conversion rates of 26.3-35.6% from exogenous linoleic acid and alpha-linolenic acid substrates, respectively. Fatty acid profiles of GC analysis from transgenic Saccharomyces cerevisiae strain INVSc1, overview
A0A1S5R926
gene DELTA6fad_a, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae; gene DELTA6fad_b, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae; gene DELTA6fad_c, several variants, genetic structure, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae
D2KE02, D2KE03, Q6SES0
gene desD, functional analysis of the Spirulina promoter in Escherichia coli, the -10 sequence, TATAAT, located at -33 bp relative to the translation start site, is essential for D6D promoter function, an ‘AT-rich inverted repeat’ -192 to -164 serves as a target-binding site for a transcriptional regulator
gene desI, recombinant expression in Saccharomyces
gene fads2, DNA and amino acid sequence determination and analysis, cloning of two alternative splicing transcripts named fads2-AS1 and fads2-AS2, quantitative real-time PCR expression analysis, recombinant expression in yeast, which shows fully functional DELTA6 desaturation activity toward C18 polyunsaturated fatty acid substrates, without residual DELTA5 and DELTA8 desaturase activities
A0A0A1E9M5
gene FADS2, rs174616 genotype, genotyping
-
gene FADS6, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain INVSc1, subcloning in Escherichia coli Top 10 cells
gene fadsd6, real-time PCR enzyme expression and copy number analysis, recombinant expression in Danio rerio. The eicosapentaenoic acid (EPA) content is 1.9fold higher in F2 transgenic delta-6 desaturase transgenic zebrafish than in wild-type fish, the fish exhibiting FADS6 enzyme expression are bigger than wild-type fish. henotype, overview. By 48 h post-infection with Vibrio alginolyticus, expression of IL-10 gene is 3.3fold higher in delta-6 desaturase transgenic fish than in wild-type fish. Expression of TLR1, TLR3, TLR4, TRAM, and NF-kappaB is also significantly different between the transgenic and wild-type fish
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gene fat-3, quantitative real-time PCR expression analysis, overview
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gene Md6, DNA and amino acid sequence determination and analysis, Md6 promoter analysis in the 5'-upstream sequence, gene Md6 contains several eukaryotic fundamental transcription regulatory elements, sequence comparisons, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1, the enzyme expression renders the yeast cells capable of converting linolenic acid to gamma-linolenic acid. The activity of DELTA6-desaturase increases by 2.8fold and 2.5fold when the mutant eMd6 sequence is placed upstream of -434 with forward or reverse orientations, respectively. The wild-type promoter of Md6 from Mucor sp. is a very strong promoter for DELTA6-desaturase and the sequence between -919 to -784 bp is an enhancer for DELTA6-desaturase activity
gene ObD6Des, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression in Saccharomyces cerevisiae strain W303-a
gene PiDesD6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Saccharomyces cerevisiae strain W303, real-time quantitative PCR expression analysis
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human sequence determined
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plasmid coding for rat delta6-desaturase constructed using pCMV for expression in mammalian cells, rat delta6-desaturase sequence, GenBank accession number AB02 1980 PCR amplified, expressed by transiently transforming COS-7 cells
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recombinant overexpression of MpFADS6 in a uracil-auxotrophic Mortierella alpina strain to enhance eicosapentaenoic acid production in Mortierella alpina by favoring the omega3 pathway, using the Agrobacterium tumefaciens-mediated transformation method. The expression of MpFADS6 results in a 26.2fold increase in EPA production compared to wild-type Mortierella alpina
RT-PCR analysis of INS-1 beta-cell content of mRNA, primer pairs basedon known rat sequence
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Saccharomyces cerevisiae transformed with fungal DELTA6-desaturase gene
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transformed into Agrobacterium tumefaciens and expressed in Glycine max
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