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1.14.19.13: acyl-CoA 15-desaturase

This is an abbreviated version!
For detailed information about acyl-CoA 15-desaturase, go to the full flat file.

Word Map on EC 1.14.19.13

Reaction

(9Z,12Z)-hexadeca-9,12-dienoyl-CoA
+
reduced acceptor
 +
O2
=
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA
 +
acceptor
 + 2 H2O

Synonyms

bifunctional DELTA12/DELTA15-fatty acid desaturase, CpFAD2, CpFAD3, CsFAD3, CSFAD3A, DELTA 15 desaturase, DELTA-12/DELTA-15 desaturase, DELTA12-desaturase, DELTA12-fatty acid desaturase, DELTA15 desaturase, DELTA15 fatty acid desaturase, DELTA15-fatty acid desaturase, DELTA15D, DES3, EhDES15, FAD3, FAD3-1, FAD3-2, FAD3-3, FAD3A, linoleate DELTA15desaturase, More, omega-3 desaturase, RKD12

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.13 acyl-CoA 15-desaturase

Cloned

Cloned on EC 1.14.19.13 - acyl-CoA 15-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae InvSc1 cells
-
expressed in Yarrowia lipolytica
-
gene CsFAD3, variant FAD3-1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview
gene CsFAD3, variant FAD3-2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview
gene CsFAD3, variant FAD3-3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview
gene EhDES15, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenic analysis, a codon-optimized EhDES15 gene lacking the 74 amino acid peptide serving as putative bipartite signal and transit peptides at the N-terminus is synthesized and expressed heterologously Synechocystis sp. PCC 6803 cells under control of the trc promoter leading to increased level of n-3 fatty acid species,which are produced at only low levels in wild-type cyanobacterial cells grown at 30°C, semi-quantitative PCR expression analysis. Expression of gene EhDES15 in a Synechocystis mutant lacking the desB gene causes accumulation of 18:3n-3 and 18:4n-3 in the transformed cells
gene FAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of a range of DELTA12- and DELTA15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis
gene FAD3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Saccharomyces cerevisiae strain BY4741 supplemented with linoleic acid (DELTA9,DELTA12-18:2) and hexadecadienoic acid (DELTA9,DELTA12-16:2) leads to accumulation of DELTA15-PUFAs, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3) results in production of DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis
gene FAD3A, quantitative RT-PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae, CSFAD3A produces 18:2DELTA9,15 from endogenous 18:1DELTA9 when expressed in Saccharomyces cerevisiae
gene RKD12, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae