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1.14.19.1: stearoyl-CoA 9-desaturase

This is an abbreviated version!
For detailed information about stearoyl-CoA 9-desaturase, go to the full flat file.

Word Map on EC 1.14.19.1

Reaction

stearoyl-CoA
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
oleoyl-CoA
+ 2 ferricytochrome b5 + 2 H2O

Synonyms

ACOD4, acyl coenzyme A desaturase, acyl-CoA desaturase, delta-9 desaturase, DELTA9 desaturase, DELTA9 fatty acid desaturase, DELTA9 terminal desaturase, DELTA9-acyl CoA desaturase, DELTA9-desaturase, DELTA9-fatty-acid desaturase, DELTA9D, DesA3, desaturase 9, Dpu-DELTA9-KPSE, EC 1.14.99.5, eicosatrienoyl-CoA desaturase, FAD9, fatty acid 9-desaturase, fatty acid DELTA9-desaturase, fatty acid desaturase, fatty acyl CoA DELTA9-desaturase, fatty acyl DELTA9-desaturase, fatty acyl-CoA desaturase, hSCD1, hSCD5, long-chain fatty acid DELTA9-desaturase, membrane-bound stearoyl-CoA DELTA9 desaturase, NPVE desaturase, palmitoyl CoA desaturase, palmitoyl-CoA desaturase, SCD, SCD-1, SCD-2, SCD-3, SCD-4, SCD1, SCD2, SCD3, SCD4, SCD5, stearoyl CoA desaturase, stearoyl coenzyme A desaturase, stearoyl coenzyme-A desaturase 1, stearoyl-ACP DELTA9 desaturase, stearoyl-CoA (DELTA8) desaturase, stearoyl-CoA desaturase, stearoyl-CoA desaturase 1, stearoyl-CoA desaturase 2, stearoyl-CoA desaturase enzyme-1, stearoyl-CoA desaturase-1, stearoyl-CoA desaturase-2, stearoyl-CoA desaturase-3, stearoyl-CoA desaturase-4, stearoyl-CoA desaturase-5, stearoyl-CoA desaturase1, stearoyl-CoA desaturase2, stearoyl-coenzyme A desaturase, stearoyl-coenzyme A desaturase-1, stearyl coenzyme A desaturase, stearyl-CoA desaturase, TkSCD-1, TkSCD-2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.1 stearoyl-CoA 9-desaturase

Purification

Purification on EC 1.14.19.1 - stearoyl-CoA 9-desaturase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, gel filtration, ultra filtration, ion-exchange
-
anionic and nonionic detergents, solubilization and ion-exchange
-
different fractions by sonication and centrifugation, ion-exchange
-
ion-exchange
-
near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview
preparation of 11 fractions, ion-exchange, gel filtration
-
recombinant isozyme SCD1 from Saccharomyces cerevisiae monounsaturated fatty acid auxotroph L8-14C, incorporation of zinc instead of iron into the protein is likely an artifact of protein overexpression, and zinc remains the predominant metal species even when the growth media and purification solutions are supplemented with iron
recombinant isozyme SCD1 partially from transfected HEL-293 cells by microsome preparation
recombinant isozyme SCD2 partially from transfected HEL-293 cells by microsome preparation
recombinant isozyme SCD3 partially from transfected HEL-293 cells by microsome preparation
several extractions besides ion-exchange and gel filtration
-
sucrose gradient, filtration
-