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1.14.18.6: 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

This is an abbreviated version!
For detailed information about 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase, go to the full flat file.

Word Map on EC 1.14.18.6

Reaction

a phytoceramide
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
a (2'R)-2'-hydroxyphytoceramide
 + 2 ferricytochrome b5 +
H2O

Synonyms

FA2H, AtFAH1, FA2H, FAH1, fatty acid 2-hydroxylase, fatty acid 2-hydroxylase 1, fatty acid alpha-hydroxylase, SCS7, Scs7p, scScs7p, sphingolipid alpha-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.6 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

Crystallization

Crystallization on EC 1.14.18.6 - 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
microbatch-under-oil method, using 20% polyethylene glycol 3350 (w/v), 100 mM HEPES, pH 7.0, 300 mM ammonium sulfate
purified recombinant wild-type scScs7p and mutant DELTA95scScs7p, microbatch-under-oil method, micxing of 0.002 ml of protein solution at 2.5 or 3.3 mg/ml with 0.002 ml of crystallization solution containing 10% PEG 2000, 100 mM sodium citrate, pH 5.6, and 50 mM ammonium sulfate, or 20% w/v PEG 3350, 100 mM HEPES, pH 7.0, and 300 mM ammonium sulfate, and then covering the drop with 0.05 ml of mineral oil, 2-7 days, 14°C, soaking in heavy atom solution saturated with K2HgI4, X-ray diffraction structure determination and analysis at 2.6-6.0 A resolution