1.14.18.3: methane monooxygenase (particulate)

This is an abbreviated version!
For detailed information about methane monooxygenase (particulate), go to the full flat file.

Reaction

Synonyms

copper-containing membrane monooxygenase, copper-containing membrane-bound monooxygenase, CuMMO, membrane-associated methane monooxygenase, membrane-bound methane monooxygenase, membrane-embedded methane monooxygenase, methane hydroxylase, mMMO, MMO, particulate methane mono-oxygenase, particulate methane monooxygenas, particulate methane monooxygenase, particulate methane monooxygenase A, particulate methane-oxidizing complex, particulate MMO, PMH, pMMO, pMMO hydroxylase, pMMO-H, pMMO1, pMMO2, PmoA, PmoB, sMMO, soluble methane monooxygenase, spmoB

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.18.3 methane monooxygenase (particulate)

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Results	in table
10	Activating Compound
572	AA Sequence
7	Application
13	Cloned(Commentary)
48	Cofactor
9	Crystallization (Commentary)
14	Expression
37	General Information
4	General Stability
31	Inhibitors
1	Ki Value [mM]
91	Localization
91	Metals/Ions
61	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
765	Organism
1	Oxidation Stability
3	Pathway
48	PDB ID
7	pH Optimum
1	pH Stability
15	Protein Variants
20	Purification (Commentary)
9	Reaction
127	Reference
1	Renatured (Commentary)
4	Source Tissue
19	Specific Activity [micromol/min/mg]
6	Storage Stability
184	Substrates and Products (Substrate)
60	Subunits
250	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]

Molecular Weight

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Molecular Weight on EC 1.14.18.3 - methane monooxygenase (particulate)

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

100000

2 entries

200000

Methylococcus capsulatus

-

non-denaturing PAGE

660351

21000

Methylosinus trichosporium

-

1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE

716110

22000

Methylococcus capsulatus

-

1 * 47000 + 1 * 24000 + 1 * 22000, X-ray crystallography

684112

220000

Methylococcus capsulatus

-

purified pMMO-detergent complex, gel filtration

716108

23000

6 entries

24000

2 entries

25000

3 entries

26000

3 entries

27000

6 entries

28328

2 entries

28376

Methylococcus capsulatus

-

1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence

716108

29063

Methylococcus capsulatus

-

1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence

716108

29073

Methylococcus capsulatus

-

1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry

716108

29733

Methylococcus capsulatus

-

1 * 42785 + 1 * 29733 + 1 * 28328, MALDI-TOF mass spectrometry

684114

300000

Methylococcus capsulatus

G1UBD1; Q607G3

alpha3beta3gamma3 trimer comprising three copies each of the pmoB (alpha), pmoA (beta), and pmoC (gamma) subunits

744325

390000

Methylococcus capsulatus

-

gel filtration

671965

40000

Methylosinus trichosporium

-

1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE

716110

42785

2 entries

42786

Methylococcus capsulatus

-

1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence

716108

45000

4 entries

450000

Methylocystis sp.

-

about, enzyme complex, native PAGE

745305

47000

6 entries

660000

Methylococcus capsulatus

-

solubilized enzyme, gel filtration

672301

94000

Methylococcus capsulatus

-

pMMO

438952

98000

Methylococcus capsulatus

G1UBD1; Q607G3

about

744405

99000

3 entries

100000

Methylococcus capsulatus

G1UBD1; Q607G3

about

745305

100000

Methylococcus capsulatus

-

apo-protein

716108

23000

Methylococcus capsulatus

-

1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE

438952

23000

Methylococcus capsulatus

-

1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview

671965

23000

Methylococcus capsulatus

-

x * 47000 + x * 26000 + x * 23000, SDS-PAGE

672301

23000

Methylococcus capsulatus

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942, 716108, 728079

23000

Methylomicrobium album

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

23000

Methylocystis sp.

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

24000

Methylococcus capsulatus

-

1 * 47000 + 1 * 24000 + 1 * 22000, X-ray crystallography

684112

24000

Methylosinus trichosporium

-

1 * 40000 + 1 * 24000 + 1 * 21000, SDS-PAGE

716110

25000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE

438944

25000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, SDS-PAGE, pMMOH

690034

25000

Methylococcus capsulatus

-

pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE

702501

26000

Methylococcus capsulatus

-

1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE

438952

26000

Methylococcus capsulatus

-

1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview

671965

26000

Methylococcus capsulatus

-

x * 47000 + x * 26000 + x * 23000, SDS-PAGE

672301

27000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE

438944

27000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, SDS-PAGE, pMMOH

690034

27000

Methylococcus capsulatus

-

pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE

702501

27000

Methylococcus capsulatus

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942, 716108, 728079

27000

Methylomicrobium album

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

27000

Methylocystis sp.

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

28328

Methylococcus capsulatus

-

1 * 42785 + 1 * 29733 + 1 * 28328, MALDI-TOF mass spectrometry

684114

28328

Methylococcus capsulatus

-

1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry

716108

42785

Methylococcus capsulatus

-

1 * 42785 + 1 * 29733 + 1 * 28328, MALDI-TOF mass spectrometry

684114

42785

Methylococcus capsulatus

-

1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry

716108

45000

Methylococcus capsulatus

-

1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE

438952

45000

Methylococcus capsulatus

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942, 716108, 728079

45000

Methylomicrobium album

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

45000

Methylocystis sp.

-

1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE

438942

47000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE

438944

47000

Methylococcus capsulatus

-

1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview

671965

47000

Methylococcus capsulatus

-

x * 47000 + x * 26000 + x * 23000, SDS-PAGE

672301

47000

Methylococcus capsulatus

-

1 * 47000 + 1 * 24000 + 1 * 22000, X-ray crystallography

684112

47000

Methylococcus capsulatus

-

1 * 47000 + 1 * 27000 + 1 * 25000, SDS-PAGE, pMMOH

690034

47000

Methylococcus capsulatus

-

pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE

702501

99000

Methylococcus capsulatus

-

-

702501

99000

Methylococcus capsulatus

-

SDS-PAGE

690034

99000

Methylococcus capsulatus

-

pMMO, mass spectrometry

438944