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1.14.18.3: methane monooxygenase (particulate)

This is an abbreviated version!
For detailed information about methane monooxygenase (particulate), go to the full flat file.

Word Map on EC 1.14.18.3

Reaction

methane
+
quinol
+
O2
=
methanol
+
quinone
+
H2O

Synonyms

copper-containing membrane monooxygenase, copper-containing membrane-bound monooxygenase, CuMMO, membrane-associated methane monooxygenase, membrane-bound methane monooxygenase, membrane-embedded methane monooxygenase, methane hydroxylase, mMMO, MMO, particulate methane mono-oxygenase, particulate methane monooxygenas, particulate methane monooxygenase, particulate methane monooxygenase A, particulate methane-oxidizing complex, particulate MMO, PMH, pMMO, pMMO hydroxylase, pMMO-H, pMMO1, pMMO2, PmoA, PmoB, sMMO, soluble methane monooxygenase, spmoB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.3 methane monooxygenase (particulate)

Crystallization

Crystallization on EC 1.14.18.3 - methane monooxygenase (particulate)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme pMMO crystal structure analysis, PDB ID 3RGB
heterotrimer of three subunits, pmoA, pmoB, and pmoC, with a solvent-exposed domain above the trans-membrane domain, which consists of alpha-helices
-
to 2.8 A resolution
purified native enzyme, sitting drop vapour diffusion method, mixing of 0.0025 ml of 20 mg/ml pMMO protein, and 0.0005 ml of 85 mM n-octyl beta-D-thiomaltoside with 0.002 ml of 10% PEG 3000, 200 mM magnesium formate dihydrate, 100 mM sodium cacodylate trihydrate, pH 6.5, at room temperature, 1-4 weeks, for the complex structure, the crystals are soaked in Zn2+ solution, X-ray diffraction structure determination and analysis at 2.6 A resolution
-
sitting drop vapor diffusion method, using 0.1 M MES, pH 6.5, 18% (w/v) PEG 750 MME, 0.03 M glycyl glycine, 0.01 M ZnSO4, at 22°C
-
crystal structure analysis, PDB ID 3CHX, structure modeling
-
hanging drop vapor diffusion method
-
to 3.9 A resolution
-