1.14.18.2: CMP-N-acetylneuraminate monooxygenase
This is an abbreviated version!
For detailed information about CMP-N-acetylneuraminate monooxygenase, go to the full flat file.
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 +
Synonyms
CMAH, CMP Neu5Ac hydroxylase, CMP-N-acetylneuraminate hydroxylase, CMP-N-acetylneuraminate,NAD(P)H:oxygen oxidoreductase (hydroxylating), CMP-N-acetylneuraminic acid hydroxylase, CMP-Neu5Ac hydroxylase, CMP-NeuAc hydroxylase, cytidine monophosphate-N-acetylneuraminic acid hydroxylase, cytidine monophospho-N-acetylneuraminic acid hydroxylase, EC 1.14.13.45, oxygenase, cytidine monophosphoacetylneuraminate mono-, pcmah
ECTree
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Cloned
Cloned on EC 1.14.18.2 - CMP-N-acetylneuraminate monooxygenase
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DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping and genotype-phenotype correlation analysis, genetic variant c.364C>T is highly associated with blood type AB in Ragdoll cats and, to a lesser degree, with type AB in random bred cats, it is associated with the expression of both antigens (N-glycolylneuraminic acid and N-acetylneuraminic acid) on the red blood cell membrane
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DNA and amino acid sequence determination of wild-type and mutant alleles, genotyping and expression analysis, overview
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expressed in pig kidney PK15 cells and human vascular endothelial ECV304 cells
full-length CMAH cDNA is cloned from umbilical cord blood mononuclear cells and HeLa cells into the pGEM-T easy vector for sequencing, GFP- or FLAG-tagged constructs are prepared using the vectors pFLAG-CMV-2 and pEGFP-C2/pEGFP-C1
C1K3L2
functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production
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gene CMAH, genotyping, genetic structure, and phylogenetic analysis. Haplotype inference, which is focused on non-synonymous single-nucleotide polymorphisms reveals that eight haplotypes carry one to four mutations in CMAH, and all cats with type B (n = 34) and AB (n = 2) blood carry two alleles derived from the mutated CMAH gene