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1.14.18.2: CMP-N-acetylneuraminate monooxygenase

This is an abbreviated version!
For detailed information about CMP-N-acetylneuraminate monooxygenase, go to the full flat file.

Word Map on EC 1.14.18.2

Reaction

CMP-N-acetylneuraminate
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
CMP-N-glycoloylneuraminate
+ 2 ferricytochrome b5 +
H2O

Synonyms

CMAH, CMP Neu5Ac hydroxylase, CMP-N-acetylneuraminate hydroxylase, CMP-N-acetylneuraminate,NAD(P)H:oxygen oxidoreductase (hydroxylating), CMP-N-acetylneuraminic acid hydroxylase, CMP-Neu5Ac hydroxylase, CMP-NeuAc hydroxylase, cytidine monophosphate-N-acetylneuraminic acid hydroxylase, cytidine monophospho-N-acetylneuraminic acid hydroxylase, EC 1.14.13.45, oxygenase, cytidine monophosphoacetylneuraminate mono-, pcmah

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.2 CMP-N-acetylneuraminate monooxygenase

Cloned

Cloned on EC 1.14.18.2 - CMP-N-acetylneuraminate monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, genotyping and genotype-phenotype correlation analysis, genetic variant c.364C>T is highly associated with blood type AB in Ragdoll cats and, to a lesser degree, with type AB in random bred cats, it is associated with the expression of both antigens (N-glycolylneuraminic acid and N-acetylneuraminic acid) on the red blood cell membrane
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DNA and amino acid sequence determination of wild-type and mutant alleles, genotyping and expression analysis, overview
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expressed in pig kidney PK15 cells and human vascular endothelial ECV304 cells
expression in Cos-1 cells
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expression in Escherichia coli
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full-length CMAH cDNA is cloned from umbilical cord blood mononuclear cells and HeLa cells into the pGEM-T easy vector for sequencing, GFP- or FLAG-tagged constructs are prepared using the vectors pFLAG-CMV-2 and pEGFP-C2/pEGFP-C1
C1K3L2
functional recombinant expression of secretory His-tagged enzyme, containing an N-terminal secretion signal peptide, in Spodoptera frugiperda Sf9 cells using the baculovirus infection method, large scale enzyme production
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gene CMAH, genotyping, genetic structure, and phylogenetic analysis. Haplotype inference, which is focused on non-synonymous single-nucleotide polymorphisms reveals that eight haplotypes carry one to four mutations in CMAH, and all cats with type B (n = 34) and AB (n = 2) blood carry two alleles derived from the mutated CMAH gene
gene CMAH, recombinant expression in HEK-293T cells
generation of bi-allelic GGTA1 and CMAH double-knockout cells
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