1.14.18.1: tyrosinase

This is an abbreviated version!
For detailed information about tyrosinase, go to the full flat file.

Word Map on EC 1.14.18.1

Reaction

2 L-dopa +

O2
= 2 dopaquinone + 2 H2O

Synonyms

AbPPO1, AbPPO4, AbTYR, aurone synthase, catalase-phenol oxidase, catechol oxidase, catecholase, CATPO, chlorogenic acid oxidase, chlorogenic oxidase, cresolase, cresolase/monophenolase, CsPPO, CZA14Tyr, deoxy-tyrosinase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, EC 1.10.3.1, Hc-derived phenoloxidase, Hc-phenoloxidase, HcPO, HdPO, hemocyanin-derived phenoloxidase, jrPPO1, jrTYR, L-DOPA monophenolase, L-DOPA oxidase, L-DOPA:oxygen oxidoreductase, L-tyrosine hydroxylase, MdPPO1, melC2, MelC2 tyrosinase, met-tyrosinase, monophenol dihydroxyphenylalanine:oxygen oxidoreductase, monophenol monooxidase, monophenol monooxygenase, monophenol monoxygenase, monophenol oxidase, monophenol oxygen oxidoreductase, monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase, monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, monophenol, L-Dopa: oxidoreductase, monophenol, L-DOPA: oxygen oxidoreductase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:O2 oxidoreductase, monophenol, o-diphenol:oxygen oxido-reductase, monophenol, o-diphenol:oxygen oxidoreductase, monophenol, polyphenol oxidase, monophenol: dioxygen oxidoreductases, hydroxylating, monophenolase, monphenol mono-oxgenase, More, mTyr, murine tyrosinase, mushroom tyrosinase, mushroom tyrosine, N-acetyl-6-hydroxytryptophan oxidase, o-diphenol oxidase, o-diphenol oxidoreductase, o-diphenol oxygen oxidoreductase, o-diphenol: O2 oxidoreductase, o-diphenol: oxidoreductase, o-diphenol:O2 oxidoreductase, o-diphenol:oxygen oxidoreductase, o-diphenolase, OCA1, Orf13, oxygen oxidoreductase, phenol oxidase, phenol oxidases, phenolase, phenoloxidase, PO, polyaromatic oxidase, polyphenol oxidase, polyphenol oxidase 3, polyphenol oxidase 4, polyphenol oxidase B, polyphenolase, polyphenoloxidase, PotPPO, PPO, PPO 3, PPO B, PPO1, PPO2, PPO3, pro-PO III, prophenoloxidase III, pyrocatechol oxidase, SPRTyr, ST94, ST94t, tryosinase, tryrosinase, TY, tyr, TYR1, TYR2, tyrA, TyrBm, tyrosinase, tyrosinase 2, tyrosinase 4, tyrosinase diphenolase, tyrosine-dopa oxidase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.1 tyrosinase

pH Stability

pH Stability on EC 1.14.18.1 - tyrosinase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 10
-
PPO exhibits a gradual increase in activity from pH 2.0-4.5, PPO exhibits a gradual decline in activity at pH above 4.5 with a slight recovery at pH 10.0
698434
2.5 - 3.5
-
the enzyme is most unstable at pH 2.5-3.5 retaining less than 10% of original activity after 2 h
712619
3 - 10
-
stable between pH 4.0 and 10.0 retaining more than 80% of its activity in the pH range of 5.0-9.0, PPO is rapidly inactivated by the pH below 5.0, the activity of recombinant PPO gradually increases with pH value in the range of 5-7, the activity decreases rapidly below and above pH 7.5, more than 70% of the optimal activity is kept between pH 7.5 and 10.0, after incubating at pH 11.0 for 1 h the recombinant PPO still retains 74% of its activity whereas at pH 3.0 it is left with only 39.4%
698038
3 - 9
-
the enzyme is extremely stable between pH 3.0 and 9.0 after 24 h incubation at 4°C retaining at least 85% of its original activity
712053
3 - 8
-
isozyme PPO III, 90% activity after 18 h at pH 3.0
636356
3
purified native enzyme, loss of 50% activity
745110
3 - 10
-
partially purified enzyme, stable, 90% of maximal activity within this range
675164
3
-
4°C, 24 h, more than 80% loss of activity
636371
3.5 - 4.5
-
optimal stability at pH 4.5, less stable above pH 6.0
636382
4 - 7
purified native enzyme, stable at
745110
4.5 - 10
-
enzyme SPRTyr, stable at
745561
5 - 10
in the pH-range from pH 5-10 the enzyme retains more than 50% of its activity
746472
5 - 8
-
the soluble enzyme shows half-lives of about 11 min, 35 min, and 3 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively. The cross-linked enzyme aggregates show half-lives of about 11 min, 35 min, and 1 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively
712534
5 - 10
-
isozyme PPO I, 90% activity after 18 h at pH 10.0
636356
5 - 7
-
purified enzyme, high stability in the pH range
746341
5
-
1 h, purified recombinant enzyme, loss of 50% activity
673560
5.5 - 9
-
most stable at pH 7.0, more than 90% of the initial activity remains after 24 h incubation at pH 7, catalase retains more than 60% of its activity at low (pH 5.5) and high (pH 9) values
684646
6 - 8
-
enzyme is stable in the pH range of 6.0-8.0
687243
6
-
4 days at 2°C, 70% inactivation
636382
6 - 7.5
-
more than 50% activity is retained during storage for 72 h at pH 6.0-7.5
712619
6 - 9.5
-
80% activity after 1 h incubation
636370
6
-
4°C, 20 h, about 75% loss of activity below
636443
6.5 - 11
4°C, 24 h, less than 10% loss of activity; 4°C, 24 h, less than 10% loss of activity
658626
6.5 - 7.4
-
partially purified enzyme, stable at
674018
6.5
-
4°C, 24 h, unstable
636371
6.5 - 10
-
enzyme CZA14Tyr, stable at
745561
7
-
pH 7.0, the enzyme retains the diphenolase activity 80% at 4°C and 40% at 30°C. The enzyme loses diphenolase activity at all pH value sat 30°C after 5 day incubation
728714
7 - 9.5
-
4°C, 24 h, cuticle-bound enzyme is stable
636341
7
-
stable at
673560
8 - 11
B2Z3P7
TyrA exhibits optimal monophenol and diphenol oxidase activities under basic conditions (pH above 8.0), when the pH is higher than 10.0 the enzyme starts to lose its activity quickly, it loses nearly 50% of activity after 12 h at pH 11.0. Within the pH range of 4.0-10.0, unlike the endogenous enzyme, the recombinant enzyme shows good stability from neutral to moderately alkaline pH, while it is unstable at acidic conditions.
695821
8 - 10
-
more than 80% of maximal activity is maintained between pH 9.0 and pH 10.0, high activity in the pH range of 8.0-10.0
745474
8.5 - 10
-
4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0
636443
9
purified native enzyme, loss of 70% activity
745110
11
-
4°C, 20 h, about 75% loss of activity above
636443
additional information