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1.14.17.1: dopamine beta-monooxygenase

This is an abbreviated version!
For detailed information about dopamine beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.17.1

Reaction

dopamine
+ 2 ascorbate +
O2
=
noradrenaline
 + 2 monodehydroascorbate +
H2O

Synonyms

3,4-dihydroxy-phenylethylamine, ascorbate: oxygen oxidoreductase (3-hydroxylating), 3,4-dihydroxyphenethylamine beta-oxidase, 3,4-dihydroxyphenylethylamine beta-hydoxylase, 4-(2-aminoethyl)pyrocatechol beta-oxidase, DbetaH, DbetaM, DBH, DBM, Dopa beta-hydroxylase, dopamine beta hydroxylase, dopamine beta-hydrolase, dopamine beta-hydroxylase, dopamine beta-mono-oxygenase, dopamine beta-monooxygenase, dopamine beta-oxidase, dopamine hydroxylase, dopamine(3,4-dihydroxyphenethylamine)beta-mono-oxygenase, dopamine-B-hydroxylase, dopamine-beta hydroxylase, dopamine-beta-hydroxylase, dopamine-beta-monooxygenase, EC 1.14.2.1, gDBH, LvDBH, MDBH, oxygenase, dopamine beta-mono-, pDbetaH, phenylamine beta-hydroxylase, plasma DbetaH activity, plasma dopamine beta-hydroxylase, plDbetaH, SDBH, TBetaM, tyramine beta-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.17.1 dopamine beta-monooxygenase

Expression

Expression on EC 1.14.17.1 - dopamine beta-monooxygenase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
A53T Tg mice that express human A53T variant alpha-synuclein under the direction of the mouse prion protein promoter show decreased DBH expression. A53T Tg mice exhibit anxiety-like behaviors and abnormal adaptive responses following the repeated immobilization stress
alpha-SYN enters the nucleus and binds directly to the DBH promoter region. alpha-Syn atteniúates the forskolin-induced DBH upregulation in SK-N-BE(2) cells
both in adrenal medulla and stellate ganglia, single immobilization significantly increases DBH mRNA and protein levels both in wild-type and corticotropin-releasing hormone knockout mice
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Cu-negative samples have significantly higher DBM activity than Cu-positive samples in both adrenal glands (2.4fold) and medulla oblongata/pons (0.9fold), but not vas deferens. No correlation between higher DBM mRNA and lower noradrenaline in Cu-negative tissues
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DBM protein abundance in adrenal glands is higher in the Cu-negative samples compared with Cu-positive samples
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Egr1 reduces DBH promoter activity, reduction occurrs as early as 4 h and reaches maximal inhibition 16-40 h after transfection. Egr1 also reduces the expression of endogenous DBH mRNA and the induction of DBH promoter activity by cAMP. The first 247, but not 200, nucleotides of DBH promoter are sufficient for suppression
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enzyme downregulation by LvDBH-dsRNA
enzyme expression is highest in 5-weeks-old rats
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forced expression of Trim11 with Phox2b specifically increases mRNA levels of dopamine beta-hydroxylase gene in primary avian neural crest stem cell culture compared to cultures where only Phox2b is forced expressed
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forskolin, dexamethasone, ionomycin, and 17-beta-estradiol all induce a significant increase in DBH promoter activity, the most significant 15.8fold upregulation of DBH transcription is observed in forskolin-treated cells The forskolin-induced DBH upregulation in SK-N-BE(2) cells is attenuated by alpha-Syn. CRE-mediated transcriptional regulation of tyrosine hydroxylase and enzyme DBH plays a crucial role in stress response
LvDBH expression is silenced in thoracic ganglia, haemocytes and hepatopancreas of shrimp by treatment with LvDBH-dsRNA for 3 days, a decrease of DBH contents in haemocytes accompanied by decreased levels of norepinephrine and dopamine in hemolymph are observed. LvDBH expressions in thoracic ganglia, haemocytes, and hepatopancreas of shrimp transferred from 28°C to 20°C for 30 min are significant higher than those held at 28°C, hypothermal stress reduces the expression of LvDBH
transfection of 0.1 to 5 microg of cDNAs of Phox2a or Phox2b significantly increases mRNA and protein levels of DBH (up to 47%) in a concentration-dependent manner. Simultanoeus transfection with both Phox2a and Phox2b does not further increase mRNA and protein levels of DBH
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transfection of shRNAs specific to Phox2a or Phox2b genes significantly reduces mRNA and protein levels of DBH after shutdown of endogenous Phox2, which is accompanied by a decreased [(3)H]norepinephrine uptake. Reduced DBH expression caused by the shRNA specific to Phox2a can be reversed by transfection with Phox2b cDNA and vice versa. Cotransfection with both shRNAs specific to Phox2a and Phox2b genes does not further reduce mRNA and protein levels of DBH
-