1.14.16.7: phenylalanine 3-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 3-monooxygenase, go to the full flat file.

Reaction

L-phenylalanine
+
tetrahydrobiopterin
+
O2
=
3-hydroxy-L-phenylalanine
+
4a-hydroxytetrahydrobiopterin

Synonyms

PacX, phenylalanine 3-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.7 phenylalanine 3-monooxygenase

Engineering

Engineering on EC 1.14.16.7 - phenylalanine 3-monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C187F
hydroxylation rate is reduced to less than 4% compared to the rate of wild-type enzyme
C187F/T202G
hydroxylation rate is comparable to that of wild-type enzyme. Less than 8% of m-Tyr compared to p-Tyr is formed
T202G
hydroxylation rate is comparable to that of wild-type enzyme. The ratio of product p-Tyr to m-Tyr is changed to 2:1 for the T202G mutant, which represents a 60-fold increase in the preference for p-Tyr over m-Tyr formation