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1.14.16.2: tyrosine 3-monooxygenase

This is an abbreviated version!
For detailed information about tyrosine 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.2

Reaction

L-tyrosine
+
a 5,6,7,8-tetrahydropteridine
+
O2
=
L-Dopa
+
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

CAT-2, DTH1, DTH2, hTH2, L-tyrosine hydroxylase, monophenol monooxygenase, oxygenase, tyrosine 3-mono-, TH, TH1, TH2, TyrH, tyrosinase, tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase, tyrosine hydroxylase type 1, tyrosine-3-mono-oxygenase, tyrosine-3-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.2 tyrosine 3-monooxygenase

Temperature Stability

Temperature Stability on EC 1.14.16.2 - tyrosine 3-monooxygenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
5 h, without stabilizing agent, complete loss of activity
40 - 65
-
minimal aggregation occurs for wild type enzyme after heating at 40°C-45 for 60 min. A steep decrease in activity is observed between 40 and 65°C, and a complete loss of activity occurs at temperatures above 70°C
41
-
Tm-value of mutant enzyme T463M is 44.3°C
42
-
or below, all mutant enzymes are stable for at least 1 h
44
-
Tm-value of mutant enzyme T245P is 44.3°C
45
-
in presence of 2% glycerol, enzymes lose activity in time-dependent manner
46
-
Tm-value of mutant enzyme R306H is 45.8°C
48
-
Tm-value of wild-type enzyme is 48.2°C
additional information
-
thermal stability of hTH1 is increased by phosphorylation at Ser-40