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1.14.16.2: tyrosine 3-monooxygenase

This is an abbreviated version!
For detailed information about tyrosine 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.2

Reaction

L-tyrosine
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
L-Dopa
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

CAT-2, DTH1, DTH2, hTH2, L-tyrosine hydroxylase, monophenol monooxygenase, oxygenase, tyrosine 3-mono-, TH, TH1, TH2, TyrH, tyrosinase, tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase, tyrosine hydroxylase type 1, tyrosine-3-mono-oxygenase, tyrosine-3-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.2 tyrosine 3-monooxygenase

Purification

Purification on EC 1.14.16.2 - tyrosine 3-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms with different MW in adrenal medulla
-
2 kinetically distinguishable forms of the enzyme: low Km form and high Km form
-
4 isoforms, recombinant from Escherichia coli
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation and Sephacryl S-300HR gel filtration
-
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration
amylose resin column chromatography and HiPrep Q column chromatography
-
from adrenal medulla
-
from brain
-
from caudate nuclei
-
GST-Sepharose column chromatography
heparin column chromatography and Superdex 200 gel filtration
-
heparin Sepharose chromatography
heparin Sepharose chromatography, incubation with ferrous iron, Q-Sepharose chromatography
-
heparin-Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200HR gel filtration
-
Hi-TRAP heparin column
-
immobilization on polyacrylamide-based support
-
immobilized metal ion affinity chromatography (Ni2+)
-
large scale
-
more than 90% homogeneity
-
Ni-affinity chromatography
-
Ni-chelating Sepharose fast flow bead chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 200 gel filtration
one chromatography step
-
recombinant
-
recombinant enzyme from Spodoptera frugiperda
-
recombinant from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and gel filtration
-
recombinant wild-type and mutants from Escherichia coli
Talon column chromatography
-
the phosphorylated TyrH is purified by Q-Sepharose column chromatography