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1.14.16.2: tyrosine 3-monooxygenase

This is an abbreviated version!
For detailed information about tyrosine 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.2

Reaction

L-tyrosine
+
a 5,6,7,8-tetrahydropteridine
+
O2
=
L-Dopa
+
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

CAT-2, DTH1, DTH2, hTH2, L-tyrosine hydroxylase, monophenol monooxygenase, oxygenase, tyrosine 3-mono-, TH, TH1, TH2, TyrH, tyrosinase, tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase, tyrosine hydroxylase type 1, tyrosine-3-mono-oxygenase, tyrosine-3-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.2 tyrosine 3-monooxygenase

Cloned

Cloned on EC 1.14.16.2 - tyrosine 3-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 isoforms, overexpression in Escherichia coli, phosphorylation-free
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a His-tagged version expressed in Escherichia coli BL21 (DE3)
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DNA and amino acid sequence determination and analysis of wild-type enzyme and polymorphic genetic variants, expression in human chromaffin cells
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DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue
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enzyme expression by in vitro transcription-translation using a rapid translation system with enhanced Escherichia coli lysate and radio-labeled L-Met, tetrahydrobioterin increases the enzyme protein yield
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expressed in BEscherichia coli BL21-CodonPlus cells
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expressed in Escherichia coli
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expressed in Escherichia coli BL21 Codon Plus (DE3) cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli strain Rosetta (DE3) pLys and HEK-293T cells
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expressed in monkey striatum using harmless adeno-associated virus vectors and in PC-12 cells and AtT-20 cells
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expressed in SH-SY5Y cells
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expressed in SH-SY5Y, SK-N-BE(2), and IMR-32 cells
expressed in SN4741 cells
expression analysis
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expression as His-tagged protein in Escherichia coli, amino acid sequence analysis
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expression Escherichia coli BL21(DE33)
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expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
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expression in Escherichia coli BL21(DE3) as His-tag fusion protein
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expression in Escherichia coli BL21(DE3) as maltose-binding protein fusion protein
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expression in Spodoptera frugiperda cells via baculovirus expression system
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expression of an enzyme-luciferase fusion protein in MN9D cells, quantitative PCR-based enzyme expression analysis, overview
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expression of gene fragment, comprising positions -495 to +25, which have regulatory promoter function, from a pGL-Basic luciferase reporter vector in MES23.5 cells and MES23.5 alpha-synuclein-overexpressing cells, alpha-synuclein is a negative regulator of the tyrosine hydroxylase expression via trans-acting function on the TH promoter, overview
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expression of mutant enzymes H331E, H336Q, H336E, E376Q, E376H, H331E/E376H in Escherichia coli BL21
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expression of wild-type and mutant enzymes in Escherichia coli
isoform 1 hTH1 and His-tagged truncated mutant, expression in Escherichia coli
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isozyme DNA and amino acid sequence determination and analysis, RT-PCR analysis of isozyme expression in neuroblastoma tumors, in abdominal and thoracic tumours and foetal adrenal glands, using external and internal primers, 4.0fold lower expression level in adrenal glands than in abdominal tumors, overview, expression of the enzyme mutant lacking exons 2, 8, and 9 in HeLa cells
-