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1.14.16.1: phenylalanine 4-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.1

Reaction

L-phenylalanine
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
L-tyrosine
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

cePAH, DicPAH, EC 1.14.3.1, EC 1.99.1.2, HPAH, L-phenylalanine 4-hydroxylase, oxygenase, phenylalanine 4-mono-, P4H, PAH, PheH, phenylalaninase, phenylalanine 4-hydroxylase, phenylalanine hydroxylase, phenylalanine monooxygenase, PheOH, phhA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.1 phenylalanine 4-monooxygenase

Purification

Purification on EC 1.14.16.1 - phenylalanine 4-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
adult and fetal liver enzyme, monoclonal antibody affinity chromatography
-
affinity chromatography on 6,7-dimethyl-5,6,7,8-tetrahydropterin-Sepharose 4B
-
ammonium sulfate precipitation and DEAE-Sephacel gel filtration
-
ammonium sulfate, Phenyl-Sepharose, DEAE-Sepharose, recombinant enzyme
-
amylose column chromatography
distinct forms I and II differing in phosphate content and isoelectric point
-
double truncated mutant enzyme DELTAN1-102/DELTAC428-452
fetal, newborn and adult enzyme are probably identical
-
glutathione-Sepharose column chromatography and Superdex S75 gel filtration
HiTrap Q column chromatography
-
metal-free enzyme by extraction of copper with dithiothreitol
-
Ni-NTA column chromatography
-
Ni-TED 2000 column chromatography, gel filtration
phenyl Sepharose column chromatography
phenyl Sepharose column chromatography and HiTrap Q column chromatography
phenyl Sepharose column chromatography and Q Sepharose column chromatography, and Superdex 200 gel filtration
-
Phenyl-Sepharose, DEAE-Sepharose
-
polyethylene glycol, Phenyl-Sepharose, DEAE-Sepharose
-
protamine, DEAE-Sephadex, acid, DEAE-cellulose, Ultrogel, hydroxylapatite, Blue dextran-phenyl-butylamine-Sepharose
-
pteridine affinity resin, 2 non-interconvertible forms
-
recombinant catalytic core mutant DELTA 117PheH and point mutants thereof from Escherichia coli strain C41(DE3)
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
recombinant enzyme, partially purified
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography
-
recombinant His-tagged enzyme in fusion with HIV transactivator of transcription protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type, I65T, R261Q and V388M mutant enzymes, affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged, catalytically active, heterozygous enzymes with one mutated allele, from bacteria by nickel affinity chromatography, excision of the His-tag
recombinant maltose-binding protein-PAH fusion protein from Escherichia coli, cleavage of the fusion protein by factor Xa, gel filtration
Q9XYQ5
recombinant maltose-binding-protein phenylalanine hydroxylase fusion protein
-
recombinant wild-type enzyme and deletion mutants
-
recombinant wild-type S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme
-
recombinant wild-type, L348V and V388M mutant enzyme, affinity chromatography
recombinant wild-type, R270K and V388M mutant enzymes expressed in the presence and absence of glycerol
Superdex 200 gel filtration
TALON resin column chromatography and Superdex 200 gel filtration
-
TALON Superflow metal affinity resin column chromatography and Superdex 200 gel filtration
-
truncated enzyme containing the C-terminal 343 amino acids
-
using Ni-NTA chromatography
-