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1.14.16.1: phenylalanine 4-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.1

Reaction

L-phenylalanine
+
a 5,6,7,8-tetrahydropteridine
+
O2
=
L-tyrosine
+
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

cePAH, DicPAH, EC 1.14.3.1, EC 1.99.1.2, HPAH, L-phenylalanine 4-hydroxylase, oxygenase, phenylalanine 4-mono-, P4H, PAH, PheH, phenylalaninase, phenylalanine 4-hydroxylase, phenylalanine hydroxylase, phenylalanine monooxygenase, PheOH, phhA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.1 phenylalanine 4-monooxygenase

Molecular Weight

Molecular Weight on EC 1.14.16.1 - phenylalanine 4-monooxygenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000 - 110000
107000
-
fetal liver enzyme, sucrose density gradient centrifugation
110000
-
fetal liver enzyme, gel filtration
13000
-
2 * 13000 (regulatory domain 1-118), gel filtration. In the presence of phenylalanine, the protein elutes earlier from the column, consistent with a conformational change in the presence of the amino acid
150000
-
adult liver enzyme, gel filtration
156000
gel filtration
160000
-
fetal liver enzyme, gel filtration
165000
-
gel filtration
200000 - 210000
210000
small-angle X-ray scattering
240000
-
sedimentation equilibrium
25000 - 27000
-
sucrose density gradient centrifugation, gel filtration
26000
-
gel filtration, regulatory domain (amino acids 1-118)
26300
catalytic domain, calculated from amino acid sequence
275000
-
-
28000
-
1 * 28000, SDS-PAGE
29000
-
recombinant enzyme, gel filtration
30300
-
gel filtration
31000
31000 - 32400
32000 - 34000
-
holo- and apo-enzyme forms display a similar hydrodynamic volume of 32000-34000 Da, apparent molecular mass based on gel filtration
32500
-
2 * 32500, calculated from amino acid sequence
33000
-
1 * 33000, SDS-PAGE
33613
-
1 * 33613, deduced from nucleotide sequence
46200
-
x * 46200, equilibrium sedimentation
49000
50000
50400
deduced from cDNA
51000
51000 - 55000
-
monomeric form, enzyme exists as monomer, dimer and tetramer
52000
53000
-
x * 53000, recombinant enzyme, SDS-PAGE
54000
-
2 * 54000, fetal liver enzyme, SDS-PAGE
64000
-
gel filtration
94000
approximately 94000 Da, SDS-PAGE
95600
-
calculated from amino acid sequence